Clinical metagenomic next-generation sequencing (mNGS), the comprehensive analysis of
microbial and host genetic material (DNA and RNA) in samples from patients, is rapidly …

Nearly all infectious agents contain DNA or RNA genomes, making sequencing an attractive
approach for pathogen detection. The cost of high-throughput or next-generation …

We developed a metagenomic next-generation sequencing (mNGS) test using cell-free DNA
from body fluids to identify pathogens. The performance of mNGS testing of 182 body fluids …

The gold standard for clinical diagnosis of bacterial lower respiratory infections (LRIs) is
culture, which has poor sensitivity and is too slow to guide early, targeted antimicrobial …

High throughput DNA sequencing methodology (next generation sequencing; NGS) has
rapidly evolved over the past 15 years and new methods are continually being …

Nanopore DNA strand sequencing has emerged as a competitive, portable technology.
Reads exceeding 150 kilobases have been achieved, as have in-field detection and …

Genome sequencing has become a powerful tool for studying emerging infectious diseases;
however, genome sequencing directly from clinical samples (ie, without isolation and …

Agnostic metagenomic next-generation sequencing (mNGS) has emerged as a promising
single, universal pathogen detection method for infectious disease diagnostics. This …

The ongoing global novel coronavirus pneumonia COVID‐19 outbreak has engendered
numerous cases of infection and death. COVID‐19 diagnosis relies upon nucleic acid …

Molecular biology has been led by various measurement technologies, and increased
throughput has developed omics analysis. The development of massively parallel …