The interplay between chromatin, transcription factors and genes generates complex
regulatory circuits that can be represented as gene regulatory networks (GRNs). The study …

Pioneer factors are transcription factors with the unique ability to initiate opening of closed
chromatin. The stability of cell identity relies on robust mechanisms that maintain the …

Pioneer transcription factors have the intrinsic biochemical ability to scan partial DNA
sequence motifs that are exposed on the surface of a nucleosome and thus access silent …

Abstract Programmable C• G-to-G• C base editors (CGBEs) have broad scientific and
therapeutic potential, but their editing outcomes have proved difficult to predict and their …

Physical access to DNA is a highly dynamic property of chromatin that plays an essential
role in establishing and maintaining cellular identity. The organization of accessible …

Cell type-specific gene expression relies on transcription factors (TFs) binding DNA
sequence motifs embedded in chromatin. Understanding how motifs are accessed in …

Abstract Assay of Transposase Accessible Chromatin sequencing (ATAC-seq) is widely
used in studying chromatin biology, but a comprehensive review of the analysis tools has …

Following Cas9 cleavage, DNA repair without a donor template is generally considered
stochastic, heterogeneous and impractical beyond gene disruption. Here, we show that …

Abstract Transposase-Accessible Chromatin followed by sequencing (ATAC-seq) is a
simple protocol for detection of open chromatin. Computational footprinting, the search for …

Although base editors are widely used to install targeted point mutations, the factors that
determine base editing outcomes are not well understood. We characterized sequence …