Principles of genetic circuit design
JAN Brophy, CA Voigt - Nature methods, 2014 - nature.com
Cells navigate environments, communicate and build complex patterns by initiating gene
expression in response to specific signals. Engineers seek to harness this capability to …
expression in response to specific signals. Engineers seek to harness this capability to …
Customizing cell signaling using engineered genetic logic circuits
Cells live in an ever-changing environment and continuously sense, process and react to
environmental signals using their inherent signaling and gene regulatory networks …
environmental signals using their inherent signaling and gene regulatory networks …
Engineering modular and orthogonal genetic logic gates for robust digital-like synthetic biology
Modular and orthogonal genetic logic gates are essential for building robust biologically
based digital devices to customize cell signalling in synthetic biology. Here we constructed …
based digital devices to customize cell signalling in synthetic biology. Here we constructed …
Tuning the dynamic range of bacterial promoters regulated by ligand-inducible transcription factors
One challenge for synthetic biologists is the predictable tuning of genetic circuit regulatory
components to elicit desired outputs. Gene expression driven by ligand-inducible …
components to elicit desired outputs. Gene expression driven by ligand-inducible …
Modular control of multiple pathways using engineered orthogonal T7 polymerases
K Temme, R Hill, TH Segall-Shapiro… - Nucleic acids …, 2012 - academic.oup.com
Synthetic genetic sensors and circuits enable programmable control over the timing and
conditions of gene expression. They are being increasingly incorporated into the control of …
conditions of gene expression. They are being increasingly incorporated into the control of …
Modular, multi-input transcriptional logic gating with orthogonal LacI/GalR family chimeras
DL Shis, F Hussain, S Meinhardt… - ACS synthetic …, 2014 - ACS Publications
In prokaryotes, the construction of synthetic, multi-input promoters is constrained by the
number of transcription factors that can simultaneously regulate a single promoter. This …
number of transcription factors that can simultaneously regulate a single promoter. This …
Bacteria-based AND logic gate: a decision-making and self-powered biosensor
We developed a bacteria-based AND logic gate using a Pseudomonas aeruginosa lasI/rhlI
double mutant with two quorum-sensing signaling molecules as the input signals. We …
double mutant with two quorum-sensing signaling molecules as the input signals. We …
Artificial cell-cell communication as an emerging tool in synthetic biology applications
Cell-cell communication is a widespread phenomenon in nature, ranging from bacterial
quorum sensing and fungal pheromone communication to cellular crosstalk in multicellular …
quorum sensing and fungal pheromone communication to cellular crosstalk in multicellular …
Reservoir computing using DNA oscillators
This paper presents novel implementations for reservoir computing (RC) using DNA
oscillators. An RC system consists of two parts: reservoir and readout layer. The reservoir …
oscillators. An RC system consists of two parts: reservoir and readout layer. The reservoir …
Programming cells: towards an automated 'Genetic Compiler'
K Clancy, CA Voigt - Current opinion in biotechnology, 2010 - Elsevier
One of the visions of synthetic biology is to be able to program cells using a language that is
similar to that used to program computers or robotics. For large genetic programs, keeping …
similar to that used to program computers or robotics. For large genetic programs, keeping …