How enzyme promiscuity and horizontal gene transfer contribute to metabolic innovation
ME Glasner, DP Truong, BC Morse - The FEBS journal, 2020 - Wiley Online Library
Promiscuity is the coincidental ability of an enzyme to catalyze its native reaction and
additional reactions that are not biological functions in the same active site. Promiscuity …
additional reactions that are not biological functions in the same active site. Promiscuity …
Recent advances in enzyme promiscuity
RD Gupta - Sustainable Chemical Processes, 2016 - Springer
Enzyme promiscuity is defined as the capability of an enzyme to catalyze a reaction other
than the reaction for which it has been specialized. Although, enzyme is known for its …
than the reaction for which it has been specialized. Although, enzyme is known for its …
Light-emitting dehalogenases: reconstruction of multifunctional biocatalysts
R Chaloupkova, V Liskova, M Toul, K Markova… - ACS …, 2019 - ACS Publications
To obtain structural insights into the emergence of biological functions from catalytically
promiscuous enzymes, we reconstructed an ancestor of catalytically distinct, but …
promiscuous enzymes, we reconstructed an ancestor of catalytically distinct, but …
Complex Loop Dynamics Underpin Activity, Specificity, and Evolvability in the (βα)8 Barrel Enzymes of Histidine and Tryptophan Biosynthesis
Enzymes are conformationally dynamic, and their dynamical properties play an important
role in regulating their specificity and evolvability. In this context, substantial attention has …
role in regulating their specificity and evolvability. In this context, substantial attention has …
Taking advantage of promiscuity of cold-active enzymes
Cold-active enzymes increase their catalytic efficiency at low-temperature, introducing
structural flexibility at or near the active sites. Inevitably, this feat seems to be accompanied …
structural flexibility at or near the active sites. Inevitably, this feat seems to be accompanied …
Evolution of substrate specificity in a retained enzyme driven by gene loss
AL Juarez-Vazquez, JN Edirisinghe… - Elife, 2017 - elifesciences.org
The connection between gene loss and the functional adaptation of retained proteins is still
poorly understood. We apply phylogenomics and metabolic modeling to detect bacterial …
poorly understood. We apply phylogenomics and metabolic modeling to detect bacterial …
Long-term persistence of bi-functionality contributes to the robustness of microbial life through exaptation
Modern enzymes are highly optimized biocatalysts that process their substrates with
extreme efficiency. Many enzymes catalyze more than one reaction; however, the …
extreme efficiency. Many enzymes catalyze more than one reaction; however, the …
[HTML][HTML] Interaction of antitubercular drug candidates with α1-acid glycoprotein produced in pulmonary granulomas
F Zsila, S Bősze, T Beke-Somfai - International journal of biological …, 2020 - Elsevier
The intracellular pathogen Mycobacterium tuberculosis can survive and replicate within host
macrophages. Among various immunomodulatory substances, macrophages also produce …
macrophages. Among various immunomodulatory substances, macrophages also produce …
Two-step Ligand Binding in a (βα) 8 barrel enzyme: substrate-bound structures shed new light on the catalytic cycle of HisA
HisA is a (βα) 8 barrel enzyme that catalyzes the Amadori rearrangement of N′-[(5′-
phosphoribosyl) formimino]-5-aminoimidazole-4-carboxamide ribonucleotide (ProFAR) to …
phosphoribosyl) formimino]-5-aminoimidazole-4-carboxamide ribonucleotide (ProFAR) to …
Increasing metagenomic resolution of microbiome interactions through functional phylogenomics and bacterial sub-communities
A Cibrián-Jaramillo, F Barona-Gómez - Frontiers in Genetics, 2016 - frontiersin.org
The genomic composition of the microbiome and its relationship with the environment is an
exciting open question in biology. Metagenomics is a useful tool in the discovery of …
exciting open question in biology. Metagenomics is a useful tool in the discovery of …