Simultaneous extraction of DNA and RNA from Escherichia coli BL 21 based on silica-coated magnetic nanoparticles

J Wang, Z Ali, N Wang, W Liang, H Liu, F Li… - Science China …, 2015 - Springer
The extraction of nucleic acid is recognized as one of the most essential steps in molecular
biology for initiating other downstream applications such as sequencing, amplification …

De novo assembly and functional annotation of Myrciaria dubia fruit transcriptome reveals multiple metabolic pathways for L-ascorbic acid biosynthesis

JC Castro, JD Maddox, M Cobos, D Requena, M Zimic… - BMC genomics, 2015 - Springer
Background Myrciaria dubia is an Amazonian fruit shrub that produces numerous bioactive
phytochemicals, but is best known by its high L-ascorbic acid (AsA) content in fruits …

[HTML][HTML] Isolation of high-quality RNA from Platycladus orientalis and other Cupressaceae plants

E Chang, Y Zhao, Q Wei, S Shi, Z Jiang - Electronic Journal of …, 2016 - Elsevier
Platycladus orientalis has a lifespan of several thousand years in China, making it a good
plant in which to study aging at the molecular level, but this requires sufficient quantities of …

Gene expression and enzyme activities of the D-mannose/L-galactose pathway influence L-ascorbic acid content in Myrciaria dubia

JC Castro, M Cobos, JD Maddox, SA Imán, A Egoavil… - Biologia plantarum, 2015 - Springer
The aim of this work was to elucidate the molecular and biochemical mechanisms that
control L-ascorbic acid (AsA) content variation in Myrciaria dubia. The AsA was quantified by …

Enriched high‑throughput reverse transcription‑quantitative PCR template preparation without pre‑amplification

T Yang, Y Ouyang, Y Gao, D Liu… - Molecular …, 2020 - spandidos-publications.com
A cDNA template with a high concentration is required to generate a high number of copies
for accurate downstream high‑throughput reverse transcription‑quantitative PCR screening …

High Quality RNA Isolation from Leaf, Shell, Root Tissues and Callus of Hazelnut (Corylus avellana L.)

H Khosravi, A Deljou, S Rashidi Monfared - Journal of Nuts, 2017 - ijnrs.damghan.iau.ir
Extraction of high quality RNA is a critical step in molecular genetics studies. Hazelnut is one
of the most important nuts plants in the world. The presence of the taxol and other taxanes in …

Isolation and molecular cloning of genes from Myrciaria dubia “camu-camu” with potential use for biotechnological production of vitamin C

JC Castro Gómez, M Cobos Ruiz… - Plant Cell …, 2016 - pgc-snia.inia.gob.pe
Myrciaria dubia “camu-camu” is a rich source of several bioactive phytochemicals and
vitamin C (L-ascorbic acid, AsA). To gain insights about the genes involved in AsA …

Efeito de sucos de frutas amazônicas na atividade in vitro da glicoproteína-P e do citocromo P450 humanos

EMA Costa - 2016 - tede.ufam.edu.br
RESUMO O metabolismo e o transporte intracelular através das membranas celulares estão
entre os principais processos que regem a cinética de fármacos em compartimentos …

Análisis estructural y funcional del genoma de Myrciaria dubia “camu-camu” como base para su mejoramiento genético

JC Castro Gómez, A Bombarely Gómez… - 2015 - alicia.concytec.gob.pe
La amazonía peruana sobresale por su mega biodiversidad de especies vegetales. Esta
biodiversidad viene siendo aprovechada por pobladores de comunidades indígenas y …

Estrategias bioinformáticas y moleculares para aislar genes con potencial biotecnológico a partir de especies huérfanas de la ciencia

M Cobos-Ruiz, JC Castro-Gómez - Ciencia Amazónica (Iquitos), 2013 - ojs.ucp.edu.pe
La amazonía peruana es megabiodiversa y posee millones de genes con potencial
biotecnológico que debemos descubrir. Para identificarlos, aislarlos y poder aprovecharlos …