The biological diversity of the unicellular bacteria—whether assessed by shape, food,
metabolism, or ecological niche—surely rivals (if not exceeds) that of the multicellular …

Bacterial cell wall synthesis is the target for some of our most powerful antibiotics and has
thus been the subject of intense research focus for more than 50 years. Surprisingly, we still …

High-throughput CRISPR-Cas9 screens have recently emerged as powerful tools to
decipher gene functions and genetic interactions. Here we use a genome-wide library of …

The vast majority of bacteria, including human pathogens and microbiome species, lack
genetic tools needed to systematically associate genes with phenotypes. This is the major …

Genome‐wide screens have discovered a large set of essential genes in the opportunistic
human pathogen Streptococcus pneumoniae. However, the functions of many essential …

The emergence of multidrug-resistant Gram-negative bacteria underscores the need to
define genetic vulnerabilities that can be therapeutically exploited. The Gram-negative …

Owing to its wide metabolic versatility and physiological robustness, together with
amenability to genetic manipulations and high resistance to stressful conditions …

By combining advanced omics technology and computational modeling, systems biologists
have identified and inferred thousands of regulatory events and system-wide interactions of …

Engineering strategies to improve terpenoids' production in Bacillus subtilis mainly focus on
2 C-methyl-d-erythritol-4-phosphate (MEP) pathway overexpression. To systematically …

Terpenoids are natural products known for their medicinal and commercial applications.
Metabolic engineering of microbial hosts for the production of valuable compounds, such as …