Extracellular vesicle analysis

A Hendrix, L Lippens, C Pinheiro, C Théry… - Nature Reviews …, 2023 - nature.com
Cells release small, phospholipid membrane-enclosed particles, collectively referred to as
extracellular vesicles (EVs), into their surroundings to enable intercellular communication …

Computational flow cytometry: helping to make sense of high-dimensional immunology data

Y Saeys, S Van Gassen, BN Lambrecht - Nature Reviews Immunology, 2016 - nature.com
Recent advances in flow cytometry allow scientists to measure an increasing number of
parameters per cell, generating huge and high-dimensional datasets. To analyse, visualize …

MIFlowCyt‐EV: A framework for standardized reporting of extracellular vesicle flow cytometry experiments

JA Welsh, E Van Der Pol, GJA Arkesteijn… - Journal of …, 2020 - Wiley Online Library
Extracellular vesicles (EVs) are small, heterogeneous and difficult to measure. Flow
cytometry (FC) is a key technology for the measurement of individual particles, but its …

Guidelines for the use of flow cytometry and cell sorting in immunological studies

A Cossarizza, HD Chang, A Radbruch… - European journal of …, 2019 - Wiley Online Library
These guidelines are a consensus work of a considerable number of members of the
immunology and flow cytometry community. They provide the theory and key practical …

Guidelines for the use of flow cytometry and cell sorting in immunological studies*

A Cossarizza, HD Chang, A Radbruch… - European journal of …, 2017 - Wiley Online Library
From a technical point of view a flow cytometer is a light detection device capable of
detecting photons of different wavelengths over a high dynamic range. In order to achieve a …

T cell assays differentiate clinical and subclinical SARS-CoV-2 infections from cross-reactive antiviral responses

A Ogbe, B Kronsteiner, DT Skelly, M Pace… - Nature …, 2021 - nature.com
Identification of protective T cell responses against SARS-CoV-2 requires distinguishing
people infected with SARS-CoV-2 from those with cross-reactive immunity to other …

The clinical utility of basophil activation testing in diagnosis and monitoring of allergic disease

HJ Hoffmann, AF Santos, C Mayorga, A Nopp… - Allergy, 2015 - Wiley Online Library
The basophil activation test (BAT) has become a pervasive test for allergic response through
the development of flow cytometry, discovery of activation markers such as CD 63 and …

Guidelines for the use of flow cytometry and cell sorting in immunological studies

A Cossarizza, HD Chang, A Radbruch… - European journal of …, 2021 - Wiley Online Library
The third edition of Flow Cytometry Guidelines provides the key aspects to consider when
performing flow cytometry experiments and includes comprehensive sections describing …

Critical assessment of automated flow cytometry data analysis techniques

N Aghaeepour, G Finak, FlowCAP Consortium… - Nature …, 2013 - nature.com
Traditional methods for flow cytometry (FCM) data processing rely on subjective manual
gating. Recently, several groups have developed computational methods for identifying cell …

Updating the MISEV minimal requirements for extracellular vesicle studies: building bridges to reproducibility

KW Witwer, C Soekmadji, AF Hill… - Journal of …, 2017 - Taylor & Francis
An editorial describing “minimal experimental requirements for definition of extracellular
vesicles (EVs)”, or more simply “minimal information for studies of EVs (MISEV)” was …