Multiplex profiling of developmental cis-regulatory elements with quantitative single-cell expression reporters

JB Lalanne, SG Regalado, S Domcke, D Calderon… - Nature …, 2024 - nature.com
The inability to scalably and precisely measure the activity of developmental cis-regulatory
elements (CREs) in multicellular systems is a bottleneck in genomics. Here we develop a …

Multiplex profiling of developmental enhancers with quantitative, single-cell expression reporters

JB Lalanne, SG Regalado, S Domcke, D Calderon… - bioRxiv, 2022 - biorxiv.org
The inability to scalably and precisely measure the activity of developmental enhancers in
multicellular systems is a bottleneck in genomics. Here, we develop a dual RNA cassette …

Widespread Increase in Enhancer—Promoter Interactions during Developmental Enhancer Activation in Mammals

Z Chen, V Snetkova, G Bower, S Jacinto, B Clock… - BioRxiv, 2022 - biorxiv.org
Remote enhancers are thought to interact with their target promoters via physical proximity,
yet the importance of this proximity for enhancer function remains unclear. Although some …

Identification of ancestral gnathostome Gli3 enhancers with activity in mammals

S Ali, M Abrar, I Hussain, F Batool… - Development …, 2024 - Wiley Online Library
Abnormal expression of the transcriptional regulator and hedgehog (Hh) signaling pathway
effector Gli3 is known to trigger congenital disease, most frequently affecting the central …

Identification and characterization of Hand2 upstream genomic enhancers active in developing stomach and limbs

CA Ferguson, BA Firulli, M Zoia… - Developmental …, 2024 - Wiley Online Library
Background The bHLH transcription factor HAND2 plays important roles in the development
of the embryonic heart, face, limbs, and sympathetic and enteric nervous systems. To define …

[HTML][HTML] Mapping cis-and trans-regulatory target genes of human-specific deletions

T Fair, BJ Pavlovic, NK Schaefer, AA Pollen - bioRxiv, 2023 - ncbi.nlm.nih.gov
Deletion of functional sequence is predicted to represent a fundamental mechanism of
molecular evolution 1, 2. Comparative genetic studies of primates 2, 3 have identified …

A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survival

S Abassah-Oppong, M Zoia, BJ Mannion… - Nature …, 2024 - nature.com
Approximately a quarter of the human genome consists of gene deserts, large regions
devoid of genes often located adjacent to developmental genes and thought to contribute to …