Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that
restricts the optical resolution to roughly 250 nm and was previously thought to be …

Super-resolution microscopy has overcome a long-held resolution barrier—the diffraction
limit—in light microscopy and enabled visualization of previously invisible molecular details …

Stimulated emission depletion (STED) microscopy provides subdiffraction resolution while
preserving useful aspects of fluorescence microscopy, such as optical sectioning, and …

The past decade has witnessed an explosion in the use of super-resolution fluorescence
microscopy methods in biology and other fields. Single-molecule localization microscopy …

Far-field optical microscopy using focused light is an important tool in a number of scientific
disciplines including chemical,(bio) physical and biomedical research, particularly with …

Despite its short history, diffraction-unlimited fluorescence microscopy techniques have
already made a substantial imprint in the biological sciences. In this review, we describe …

Paraformaldehyde (PFA) is the most commonly used fixative for immunostaining of cells, but
has been associated with various problems, ranging from loss of antigenicity to changes in …

Various techniques have been developed and used to investigate how proteins produce
complex biological architectures and phenomena. Among these techniques, high-speed …

Stimulated emission depletion (STED) nanoscopy allows observations of subcellular
dynamics at the nanoscale. Applications have, however, been severely limited by the lack of …

Super-resolution optical imaging based on the switching and localization of individual
fluorescent molecules [photoactivated localization microscopy (PALM), stochastic optical …