Smart reprograming of plants against salinity stress using modern biotechnological tools
Climate change gives rise to numerous environmental stresses, including soil salinity.
Salinity/salt stress is the second biggest abiotic factor affecting agricultural productivity …
Salinity/salt stress is the second biggest abiotic factor affecting agricultural productivity …
Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
The development of new CRISPR–Cas genome editing tools continues to drive major
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
Strand-preferred base editing of organellar and nuclear genomes using CyDENT
Transcription-activator-like effector (TALE)-based tools for base editing of nuclear and
organellar DNA rely on double-stranded DNA deaminases, which edit substrate bases on …
organellar DNA rely on double-stranded DNA deaminases, which edit substrate bases on …
Gene Editing of CCR5 in Autologous CD4 T Cells of Persons Infected with HIV
P Tebas, D Stein, WW Tang, I Frank… - … England Journal of …, 2014 - Mass Medical Soc
Background CCR5 is the major coreceptor for human immunodeficiency virus (HIV). We
investigated whether site-specific modification of the gene (“gene editing”)—in this case, the …
investigated whether site-specific modification of the gene (“gene editing”)—in this case, the …
Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA
CD Richardson, GJ Ray, MA DeWitt, GL Curie… - Nature …, 2016 - nature.com
Targeted genomic manipulation by Cas9 can efficiently generate knockout cells and
organisms via error-prone nonhomologous end joining (NHEJ), but the efficiency of precise …
organisms via error-prone nonhomologous end joining (NHEJ), but the efficiency of precise …
Defining and improving the genome-wide specificities of CRISPR–Cas9 nucleases
SQ Tsai, JK Joung - Nature Reviews Genetics, 2016 - nature.com
CRISPR–Cas9 RNA-guided nucleases are a transformative technology for biology, genetics
and medicine owing to the simplicity with which they can be programmed to cleave specific …
and medicine owing to the simplicity with which they can be programmed to cleave specific …
A guide to genome engineering with programmable nucleases
H Kim, JS Kim - Nature Reviews Genetics, 2014 - nature.com
Programmable nucleases—including zinc-finger nucleases (ZFNs), transcription activator-
like effector nucleases (TALENs) and RNA-guided engineered nucleases (RGENs) derived …
like effector nucleases (TALENs) and RNA-guided engineered nucleases (RGENs) derived …
[HTML][HTML] Origins of programmable nucleases for genome engineering
S Chandrasegaran, D Carroll - Journal of molecular biology, 2016 - Elsevier
Genome engineering with programmable nucleases depends on cellular responses to a
targeted double-strand break (DSB). The first truly targetable reagents were the zinc finger …
targeted double-strand break (DSB). The first truly targetable reagents were the zinc finger …
Targeting DNA double-strand breaks with TAL effector nucleases
Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for
targeted mutagenesis. Here we report a new class of sequence-specific nucleases created …
targeted mutagenesis. Here we report a new class of sequence-specific nucleases created …
Genome engineering with zinc-finger nucleases
D Carroll - Genetics, 2011 - academic.oup.com
Zinc-finger nucleases (ZFNs) are targetable DNA cleavage reagents that have been
adopted as gene-targeting tools. ZFN-induced double-strand breaks are subject to cellular …
adopted as gene-targeting tools. ZFN-induced double-strand breaks are subject to cellular …