Guidelines for the purification and characterization of extracellular vesicles of parasites

C Fernandez‐Becerra, P Xander… - Journal of …, 2023 - Wiley Online Library
Parasites are responsible for the most neglected tropical diseases, affecting over a billion
people worldwide (WHO, 2015) and accounting for billions of cases a year and responsible …

Subcellular particles for characterization of host-parasite interactions

E Kozela, P Meneghetti, N Regev-Rudzki… - Microbes and …, 2024 - Elsevier
Parasitic diseases remain a major global health problem for humans. Parasites employ a
variety of strategies to invade and survive within their hosts and to manipulate host defense …

Extracellular vesicles in parasitic diseases–from pathogenesis to future diagnostic tools

SA Menezes, T Tasca - Microbes and Infection, 2024 - Elsevier
Parasitic diseases are still a major public health problem especially among individuals of
low socioeconomic status in underdeveloped countries. In recent years it has been …

Distinct immunomodulatory properties of extracellular vesicles released by different strains of Acanthamoeba

AO Costa, IAR Chagas… - Cell Biology …, 2021 - Wiley Online Library
Free living amoeba of the genus Acanthamoeba are opportunist protozoan involved in
corneal, systemic, and encephalic infections in humans. Most of the mechanisms underlying …

[HTML][HTML] Extracellular Vesicles Secreted by Acanthamoeba culbertsoni Have COX and Proteolytic Activity and Induce Hemolysis

F Sierra-López, I Castelan-Ramírez… - Microorganisms, 2023 - mdpi.com
Several species of Acanthamoeba genus are potential pathogens and etiological agents of
several diseases. The pathogenic mechanisms carried out by these amoebae in different …

[HTML][HTML] Assessing Acanthamoeba cytotoxicity: comparison of common cell viability assays

A Loufouma Mbouaka, I Lesiak-Markowicz… - Frontiers in …, 2023 - frontiersin.org
Background In vitro models for studying interactions between Acanthamoeba and host cells
are crucial for understanding the pathomechanism of Acanthamoeba and assessing …

[HTML][HTML] Isolation and morphological and molecular characterization of waterborne free-living amoebae: Evidence of potentially pathogenic Acanthamoeba and …

MM Nageeb, HEM Eldeek, RAH Attia, AA Sakla… - Plos one, 2022 - journals.plos.org
Free-living amoebae (FLA) are gaining attention due to the increasing number of related
grave central nervous system (CNS) and sight-threatening eye infections and their role as …

[HTML][HTML] Providing an in vitro depiction of microglial cells challenged with immunostimulatory extracellular vesicles of Naegleria fowleri

L Retana Moreira, A Cornet-Gomez… - Frontiers in …, 2024 - frontiersin.org
Naegleria fowleri is the causative agent of primary amoebic meningoencephalitis, a rapid
and acute infection of the central nervous system with a fatal outcome in> 97% of cases. Due …

Comprehensive characterization of extracellular vesicles produced by environmental (Neff) and clinical (T4) strains of Acanthamoeba castellanii

EG Medeiros, MR Valente, L Honorato, MS Ferreira… - Msystems, 2024 - Am Soc Microbiol
We conducted a comprehensive comparative analysis of extracellular vesicles (EVs) from
two Acanthamoeba castellanii strains, Neff (environmental) and T4 (clinical). Morphological …

[HTML][HTML] Providing an in vitro depiction of microglial cells challenged with immunostimulatory extracellular vesicles of Naegleria fowleri

LR Moreira, A Cornet-Gomez… - Frontiers in …, 2024 - ncbi.nlm.nih.gov
Naegleria fowleri is the causative agent of primary amoebic meningoencephalitis, a rapid
and acute infection of the central nervous system with a fatal outcome in> 97% of cases. Due …