Despite the availability of Cas9 variants with varied protospacer-adjacent motif (PAM)
compatibilities, some genomic loci—especially those with pyrimidine-rich PAM sequences …

Main conclusion This review provides a comprehensive overview of the CRISPR/Cas9
technique and the research areas of this gene editing tool in improving wheat quality …

Genome editing technologies hold tremendous potential in biomedical research and drug
development. Therefore, it is imperative to discover gene editing tools with superior cutting …

Programmable, RNA-guided nucleases are diverse enzymes that have been repurposed for
biotechnological applications. However, to further expand the therapeutic application of …

Discovery of the CRISPR/Cas system revolutionized biology and biomedicine in the 21st
century. Here we discuss the milestones in the development of CRISPR/Cas genome editing …

Recently, various small Cas9 orthologs and variants have been reported for use in in vivo
delivery applications. Although small Cas9s are particularly suited for this purpose, selecting …

Abstract The RNA-guided CRISPR/Cas9 system is a powerful tool for genome editing, but its
targeting scope is limited by the protospacer-adjacent motif (PAM). To expand the target …

CjCas9 is one of the smallest CRISPR-associated (Cas9) nucleases for mammalian
genome editing. However, it requires a long N 4 RYAC (R= A or G; Y= C or T) protospacer …

CRISPR-Cas9 systems have been widely harnessed for diverse genome editing
applications because of their ease of use and high efficiency. However, the large molecular …

The increasing emergence of Cas9 variants has attracted broad interest, as these variants
were designed to expand CRISPR applications. New Cas9 variants typically feature higher …