Whole-brain profiling of cells and circuits in mammals by tissue clearing and light-sheet microscopy
Tissue clearing and light-sheet microscopy have a 100-year-plus history, yet these fields
have been combined only recently to facilitate novel experiments and measurements in …
have been combined only recently to facilitate novel experiments and measurements in …
[HTML][HTML] The BRAIN initiative cell census consortium: lessons learned toward generating a comprehensive brain cell atlas
A comprehensive characterization of neuronal cell types, their distributions, and patterns of
connectivity is critical for understanding the properties of neural circuits and how they …
connectivity is critical for understanding the properties of neural circuits and how they …
Molecular basis of astrocyte diversity and morphology across the CNS in health and disease
Astrocytes, a type of glia, are abundant and morphologically complex cells. Here, we report
astrocyte molecular profiles, diversity, and morphology across the mouse central nervous …
astrocyte molecular profiles, diversity, and morphology across the mouse central nervous …
High-throughput mapping of a whole rhesus monkey brain at micrometer resolution
Whole-brain mesoscale mapping in primates has been hindered by large brain sizes and
the relatively low throughput of available microscopy methods. Here, we present an …
the relatively low throughput of available microscopy methods. Here, we present an …
Advanced CUBIC tissue clearing for whole-organ cell profiling
K Matsumoto, TT Mitani, SA Horiguchi, J Kaneshiro… - Nature protocols, 2019 - nature.com
Tissue-clearing techniques are powerful tools for biological research and pathological
diagnosis. Here, we describe advanced clear, unobstructed brain imaging cocktails and …
diagnosis. Here, we describe advanced clear, unobstructed brain imaging cocktails and …
Generation of a whole-brain atlas for the cholinergic system and mesoscopic projectome analysis of basal forebrain cholinergic neurons
X Li, B Yu, Q Sun, Y Zhang, M Ren… - Proceedings of the …, 2018 - National Acad Sciences
The cholinergic system in the brain plays crucial roles in regulating sensory and motor
functions as well as cognitive behaviors by modulating neuronal activity. Understanding the …
functions as well as cognitive behaviors by modulating neuronal activity. Understanding the …
A three-dimensional single-cell-resolution whole-brain atlas using CUBIC-X expansion microscopy and tissue clearing
A three-dimensional single-cell-resolution mammalian brain atlas will accelerate systems-
level identification and analysis of cellular circuits underlying various brain functions …
level identification and analysis of cellular circuits underlying various brain functions …
High-definition imaging using line-illumination modulation microscopy
Q Zhong, A Li, R Jin, D Zhang, X Li, X Jia, Z Ding… - Nature …, 2021 - nature.com
The microscopic visualization of large-scale three-dimensional (3D) samples by optical
microscopy requires overcoming challenges in imaging quality and speed and in big data …
microscopy requires overcoming challenges in imaging quality and speed and in big data …
Claustrum mediates bidirectional and reversible control of stress-induced anxiety responses
M Niu, A Kasai, M Tanuma, K Seiriki, H Igarashi… - Science …, 2022 - science.org
The processing of stress responses involves brain-wide communication among cortical and
subcortical regions; however, the underlying mechanisms remain elusive. Here, we show …
subcortical regions; however, the underlying mechanisms remain elusive. Here, we show …
Miniscope-LFOV: a large-field-of-view, single-cell-resolution, miniature microscope for wired and wire-free imaging of neural dynamics in freely behaving animals
Imaging large-population, single-cell fluorescent dynamics in freely behaving animals larger
than mice remains a key endeavor of neuroscience. We present a large-field-of-view open …
than mice remains a key endeavor of neuroscience. We present a large-field-of-view open …