Leveraging crosslinking mass spectrometry in structural and cell biology

A Graziadei, J Rappsilber - Structure, 2022 - cell.com
Crosslinking mass spectrometry (crosslinking-MS) is a versatile tool providing structural
insights into protein conformation and protein-protein interactions. Its medium-resolution …

Cryo-EM structure of DNA-bound Smc5/6 reveals DNA clamping enabled by multi-subunit conformational changes

Y Yu, S Li, Z Ser, H Kuang, T Than… - Proceedings of the …, 2022 - National Acad Sciences
Structural maintenance of chromosomes (SMC) complexes are essential for chromatin
organization and functions throughout the cell cycle. The cohesin and condensin SMCs fold …

Mass Spectrometry Structural Proteomics Enabled by Limited Proteolysis and Cross‐Linking

H Lu, Z Zhu, L Fields, H Zhang… - Mass Spectrometry …, 2024 - Wiley Online Library
The exploration of protein structure and function stands at the forefront of life science and
represents an ever‐expanding focus in the development of proteomics. As mass …

[HTML][HTML] xiVIEW: Visualisation of crosslinking mass spectrometry data

CW Combe, M Graham, L Kolbowski, L Fischer… - Journal of Molecular …, 2024 - Elsevier
Crosslinking mass spectrometry (MS) has emerged as an important technique for
elucidating the in-solution structures of protein complexes and the topology of protein …

Molecular structure of soluble vimentin tetramers

PJ Vermeire, AV Lilina, HM Hashim, L Dlabolová… - Scientific Reports, 2023 - nature.com
Intermediate filaments (IFs) are essential constituents of the metazoan cytoskeleton. A vast
family of cytoplasmic IF proteins are capable of self-assembly from soluble tetrameric …

Molecular architecture of the augmin complex

CA Gabel, Z Li, AG DeMarco, Z Zhang, J Yang… - Nature …, 2022 - nature.com
Accurate segregation of chromosomes during mitosis depends on the correct assembly of
the mitotic spindle, a bipolar structure composed mainly of microtubules. The augmin …

The LIKE SEX FOUR 1–malate dehydrogenase complex functions as a scaffold to recruit β-amylase to promote starch degradation

J Liu, X Wang, Z Guan, M Wu, X Wang, R Fan… - The Plant …, 2024 - academic.oup.com
In plant leaves, starch is composed of glucan polymers that accumulate in chloroplasts as
the products of photosynthesis during the day; starch is mobilized at night to continuously …

Dynamic interplay between the periplasmic chaperone SurA and the BAM complex in outer membrane protein folding

B Schiffrin, JM Machin, TK Karamanos… - Communications …, 2022 - nature.com
Correct folding of outer membrane proteins (OMPs) into the outer membrane of Gram-
negative bacteria depends on delivery of unfolded OMPs to the β-barrel assembly …

NEAT1 modulates the TIRR/53BP1 complex to maintain genome integrity

S Kilgas, A Syed, P Toolan-Kerr, ML Swift… - Nature …, 2024 - nature.com
Abstract Tudor Interacting Repair Regulator (TIRR) is an RNA-binding protein (RBP) that
interacts directly with 53BP1, restricting its access to DNA double-strand breaks (DSBs) and …

Effects of vegetation cover and aquaculture pollution on viral assemblages in mangroves sediments

Y Su, H Yu, C Gao, S Sun, Y Liang, G Liu… - Journal of Hazardous …, 2024 - Elsevier
Mangrove forests, a critical coastal ecosystem, face numerous anthropogenic threats,
particularly from aquaculture activities. Despite the acknowledged significance of viruses in …