Applications of CRISPR technologies in research and beyond

R Barrangou, JA Doudna - Nature biotechnology, 2016 - nature.com
Programmable DNA cleavage using CRISPR–Cas9 enables efficient, site-specific genome
engineering in single cells and whole organisms. In the research arena, versatile CRISPR …

Progress and prospects of CRISPR/Cas systems in insects and other arthropods

D Sun, Z Guo, Y Liu, Y Zhang - Frontiers in physiology, 2017 - frontiersin.org
Clustered regularly interspaced short palindromic repeats (CRISPR) and the CRISPR-
associated gene Cas9 represent an invaluable system for the precise editing of genes in …

Genomic adaptation to polyphagy and insecticides in a major East Asian noctuid pest

T Cheng, J Wu, Y Wu, RV Chilukuri, L Huang… - Nature ecology & …, 2017 - nature.com
The tobacco cutworm, Spodoptera litura, is among the most widespread and destructive
agricultural pests, feeding on over 100 crops throughout tropical and subtropical Asia. By …

Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing

D Chaverra-Rodriguez, VM Macias, GL Hughes… - Nature …, 2018 - nature.com
Cas9-mediated gene editing is a powerful tool for addressing research questions in
arthropods. Current approaches rely upon delivering Cas9 ribonucleoprotein (RNP) …

Development of CRISPR-Cas systems for genome editing and beyond

F Zhang - Quarterly Reviews of Biophysics, 2019 - cambridge.org
The development of clustered regularly interspaced short-palindromic repeat (CRISPR)-Cas
systems for genome editing has transformed the way life science research is conducted and …

[HTML][HTML] Insert, remove or replace: A highly advanced genome editing system using CRISPR/Cas9

SA Ceasar, V Rajan, SV Prykhozhij, JN Berman… - … et Biophysica Acta (BBA …, 2016 - Elsevier
The clustered, regularly interspaced, short palindromic repeat (CRISPR) and CRISPR
associated protein 9 (Cas9) system discovered as an adaptive immunity mechanism in …

CRISPR/Cas9 in insects: Applications, best practices and biosafety concerns

CNT Taning, B Van Eynde, N Yu, S Ma… - Journal of insect …, 2017 - Elsevier
Discovered as a bacterial adaptive immune system, CRISPR/Cas9 (clustered, regularly
interspaced, short palindromic repeat/CRISPR associated) is being developed as an …

[HTML][HTML] The evolution and diversification of oakleaf butterflies

S Wang, D Teng, X Li, P Yang, W Da, Y Zhang… - Cell, 2022 - cell.com
Oakleaf butterflies in the genus Kallima have a polymorphic wing phenotype, enabling these
insects to masquerade as dead leaves. This iconic example of protective resemblance …

Functional validation of cadherin as a receptor of Bt toxin Cry1Ac in Helicoverpa armigera utilizing the CRISPR/Cas9 system

J Wang, H Zhang, H Wang, S Zhao, Y Zuo… - Insect Biochemistry and …, 2016 - Elsevier
Cadherins have been identified as receptors of Bacillus thuringiensis (Bt) Cry1A toxins in
several lepidopteran insects including the cotton bollworm, Helicoverpa armigera …

CRISPR/Cas9 in locusts: successful establishment of an olfactory deficiency line by targeting the mutagenesis of an odorant receptor co-receptor (Orco)

Y Li, J Zhang, D Chen, P Yang, F Jiang, X Wang… - Insect biochemistry and …, 2016 - Elsevier
Locusts are important agricultural pests worldwide and regarded as study models for
entomology. However, the absence of targeted gene manipulation systems for locusts has …