Outer membrane vesicles as platform vaccine technology

L Van Der Pol, M Stork, P van der Ley - Biotechnology journal, 2015 - Wiley Online Library
Outer membrane vesicles (OMVs) are released spontaneously during growth by many Gram‐
negative bacteria. They present a range of surface antigens in a native conformation and …

The versatile β-barrel membrane protein

WC Wimley - Current opinion in structural biology, 2003 - Elsevier
The β-barrel membrane protein is found in the outer membranes of bacteria, mitochondria
and chloroplasts. Approximately 2–3% of the genes in Gram-negative bacterial genomes …

Large-scale analysis of the yeast proteome by multidimensional protein identification technology

MP Washburn, D Wolters, JR Yates - Nature biotechnology, 2001 - nature.com
We describe a largely unbiased method for rapid and large-scale proteome analysis by
multidimensional liquid chromatography, tandem mass spectrometry, and database …

Global proteomic profiling of native outer membrane vesicles derived from Escherichia coli

EY Lee, JY Bang, GW Park, DS Choi, JS Kang… - …, 2007 - Wiley Online Library
Gram‐negative bacteria constitutively secrete native outer membrane vesicles (OMVs) into
the extracellular milieu. Although recent progress in this area has revealed that OMVs are …

TiO2 Photocatalysis Damages Lipids and Proteins in Escherichia coli

G Carré, E Hamon, S Ennahar, M Estner… - Applied and …, 2014 - Am Soc Microbiol
This study investigates the mechanisms of UV-A (315 to 400 nm) photocatalysis with
titanium dioxide (TiO2) applied to the degradation of Escherichia coli and their effects on two …

[HTML][HTML] Consequences of membrane protein overexpression in Escherichia coli

S Wagner, L Baars, AJ Ytterberg, A Klussmeier… - Molecular & Cellular …, 2007 - Elsevier
Overexpression of membrane proteins is often essential for structural and functional studies,
but yields are frequently too low. An understanding of the physiological response to …

Quantitative profiling of differentiation-induced microsomal proteins using isotope-coded affinity tags and mass spectrometry

DK Han, J Eng, H Zhou, R Aebersold - Nature biotechnology, 2001 - nature.com
An approach to the systematic identification and quantification of the proteins contained in
the microsomal fraction of cells is described. It consists of three steps:(1) preparation of …

CHARMM‐GUI Martini Maker for modeling and simulation of complex bacterial membranes with lipopolysaccharides

PC Hsu, BMH Bruininks, D Jefferies… - 2017 - Wiley Online Library
A complex cell envelope, composed of a mixture of lipid types including
lipopolysaccharides, protects bacteria from the external environment. Clearly, the proteins …

Characterization and identification of vaccine candidate proteins through analysis of the group A Streptococcus surface proteome

MJ Rodríguez-Ortega, N Norais, G Bensi… - Nature …, 2006 - nature.com
We describe a proteomic approach for identifying bacterial surface-exposed proteins quickly
and reliably for their use as vaccine candidates. Whole cells are treated with proteases to …

Developing vaccines in the era of genomics: a decade of reverse vaccinology

KL Seib, X Zhao, R Rappuoli - Clinical Microbiology and …, 2012 - Wiley Online Library
Clin Microbiol Infect 2012; 18 (Suppl. 5): 109–116 Abstract Vaccines have a significant
impact on public health, and vaccinology in the era of genomics is taking advantage of new …