Outer membrane vesicles as platform vaccine technology
L Van Der Pol, M Stork, P van der Ley - Biotechnology journal, 2015 - Wiley Online Library
Outer membrane vesicles (OMVs) are released spontaneously during growth by many Gram‐
negative bacteria. They present a range of surface antigens in a native conformation and …
negative bacteria. They present a range of surface antigens in a native conformation and …
The versatile β-barrel membrane protein
WC Wimley - Current opinion in structural biology, 2003 - Elsevier
The β-barrel membrane protein is found in the outer membranes of bacteria, mitochondria
and chloroplasts. Approximately 2–3% of the genes in Gram-negative bacterial genomes …
and chloroplasts. Approximately 2–3% of the genes in Gram-negative bacterial genomes …
Large-scale analysis of the yeast proteome by multidimensional protein identification technology
We describe a largely unbiased method for rapid and large-scale proteome analysis by
multidimensional liquid chromatography, tandem mass spectrometry, and database …
multidimensional liquid chromatography, tandem mass spectrometry, and database …
Global proteomic profiling of native outer membrane vesicles derived from Escherichia coli
Gram‐negative bacteria constitutively secrete native outer membrane vesicles (OMVs) into
the extracellular milieu. Although recent progress in this area has revealed that OMVs are …
the extracellular milieu. Although recent progress in this area has revealed that OMVs are …
TiO2 Photocatalysis Damages Lipids and Proteins in Escherichia coli
G Carré, E Hamon, S Ennahar, M Estner… - Applied and …, 2014 - Am Soc Microbiol
This study investigates the mechanisms of UV-A (315 to 400 nm) photocatalysis with
titanium dioxide (TiO2) applied to the degradation of Escherichia coli and their effects on two …
titanium dioxide (TiO2) applied to the degradation of Escherichia coli and their effects on two …
[HTML][HTML] Consequences of membrane protein overexpression in Escherichia coli
S Wagner, L Baars, AJ Ytterberg, A Klussmeier… - Molecular & Cellular …, 2007 - Elsevier
Overexpression of membrane proteins is often essential for structural and functional studies,
but yields are frequently too low. An understanding of the physiological response to …
but yields are frequently too low. An understanding of the physiological response to …
Quantitative profiling of differentiation-induced microsomal proteins using isotope-coded affinity tags and mass spectrometry
An approach to the systematic identification and quantification of the proteins contained in
the microsomal fraction of cells is described. It consists of three steps:(1) preparation of …
the microsomal fraction of cells is described. It consists of three steps:(1) preparation of …
CHARMM‐GUI Martini Maker for modeling and simulation of complex bacterial membranes with lipopolysaccharides
PC Hsu, BMH Bruininks, D Jefferies… - 2017 - Wiley Online Library
A complex cell envelope, composed of a mixture of lipid types including
lipopolysaccharides, protects bacteria from the external environment. Clearly, the proteins …
lipopolysaccharides, protects bacteria from the external environment. Clearly, the proteins …
Characterization and identification of vaccine candidate proteins through analysis of the group A Streptococcus surface proteome
MJ Rodríguez-Ortega, N Norais, G Bensi… - Nature …, 2006 - nature.com
We describe a proteomic approach for identifying bacterial surface-exposed proteins quickly
and reliably for their use as vaccine candidates. Whole cells are treated with proteases to …
and reliably for their use as vaccine candidates. Whole cells are treated with proteases to …
Developing vaccines in the era of genomics: a decade of reverse vaccinology
KL Seib, X Zhao, R Rappuoli - Clinical Microbiology and …, 2012 - Wiley Online Library
Clin Microbiol Infect 2012; 18 (Suppl. 5): 109–116 Abstract Vaccines have a significant
impact on public health, and vaccinology in the era of genomics is taking advantage of new …
impact on public health, and vaccinology in the era of genomics is taking advantage of new …