Review of CRISPR/Cas9 sgRNA design tools
The adaptive immunity system in bacteria and archaea, Clustered Regularly Interspaced
Short Palindromic Repeats, CRISPR-associate (CRISPR/Cas), has been adapted as a …
Short Palindromic Repeats, CRISPR-associate (CRISPR/Cas), has been adapted as a …
Design and analysis of CRISPR–Cas experiments
A large and ever-expanding set of CRISPR–Cas systems now enables the rapid and flexible
manipulation of genomes in both targeted and large-scale experiments. Numerous software …
manipulation of genomes in both targeted and large-scale experiments. Numerous software …
Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities
The creation of genome-wide libraries for CRISPR knockout (CRISPRko), interference
(CRISPRi), and activation (CRISPRa) has enabled the systematic interrogation of gene …
(CRISPRi), and activation (CRISPRa) has enabled the systematic interrogation of gene …
Human pancreatic islet three-dimensional chromatin architecture provides insights into the genetics of type 2 diabetes
Genetic studies promise to provide insight into the molecular mechanisms underlying type 2
diabetes (T2D). Variants associated with T2D are often located in tissue-specific enhancer …
diabetes (T2D). Variants associated with T2D are often located in tissue-specific enhancer …
[HTML][HTML] Computational approaches for effective CRISPR guide RNA design and evaluation
Abstract The Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/CRISPR-
associated (Cas) system has emerged as the main technology for gene editing. Successful …
associated (Cas) system has emerged as the main technology for gene editing. Successful …
Hacking the cancer genome: profiling therapeutically actionable long non-coding RNAs using CRISPR-Cas9 screening
Long non-coding RNAs (lncRNAs) represent a huge reservoir of potential cancer targets.
Such" onco-lncRNAs" have resisted traditional RNAi methods, but CRISPR-Cas9 genome …
Such" onco-lncRNAs" have resisted traditional RNAi methods, but CRISPR-Cas9 genome …
Harnessing accurate non-homologous end joining for efficient precise deletion in CRISPR/Cas9-mediated genome editing
T Guo, YL Feng, JJ Xiao, Q Liu, XN Sun, JF Xiang… - Genome biology, 2018 - Springer
Background Many applications of CRISPR/Cas9-mediated genome editing require Cas9-
induced non-homologous end joining (NHEJ), which was thought to be error prone …
induced non-homologous end joining (NHEJ), which was thought to be error prone …
Prunus genetics and applications after de novo genome sequencing: achievements and prospects
MJ Aranzana, V Decroocq, E Dirlewanger… - Horticulture …, 2019 - academic.oup.com
Prior to the availability of whole-genome sequences, our understanding of the structural and
functional aspects of Prunus tree genomes was limited mostly to molecular genetic mapping …
functional aspects of Prunus tree genomes was limited mostly to molecular genetic mapping …
Loss of polycomb repressive complex 1 activity and chromosomal instability drive uveal melanoma progression
Chromosomal instability (CIN) and epigenetic alterations have been implicated in tumor
progression and metastasis; yet how these two hallmarks of cancer are related remains …
progression and metastasis; yet how these two hallmarks of cancer are related remains …
Dynamic chromatin regulatory landscape of human CAR T cell exhaustion
DG Gennert, RC Lynn, JM Granja… - Proceedings of the …, 2021 - National Acad Sciences
Dysfunction in T cells limits the efficacy of cancer immunotherapy. We profiled the
epigenome, transcriptome, and enhancer connectome of exhaustion-prone GD2-targeting …
epigenome, transcriptome, and enhancer connectome of exhaustion-prone GD2-targeting …