Recombinase polymerase amplification: Basics, applications and recent advances
IM Lobato, CK O'Sullivan - Trac Trends in analytical chemistry, 2018 - Elsevier
Recombinase polymerase amplification (RPA) is a highly sensitive and selective isothermal
amplification technique, operating at 37–42° C, with minimal sample preparation and …
amplification technique, operating at 37–42° C, with minimal sample preparation and …
A review of biomedical centrifugal microfluidic platforms
Centrifugal microfluidic or lab-on-a-disc platforms have many advantages over other
microfluidic systems. These advantages include a minimal amount of instrumentation, the …
microfluidic systems. These advantages include a minimal amount of instrumentation, the …
Integration of isothermal amplification methods in microfluidic devices: Recent advances
MC Giuffrida, G Spoto - Biosensors and Bioelectronics, 2017 - Elsevier
The integration of nucleic acids detection assays in microfluidic devices represents a highly
promising approach for the development of convenient, cheap and efficient diagnostic tools …
promising approach for the development of convenient, cheap and efficient diagnostic tools …
Multiplexed isothermal nucleic acid amplification
O Mayboroda, I Katakis, CK O'Sullivan - Analytical biochemistry, 2018 - Elsevier
Multiplexed isothermal amplification and detection of nucleic acid sequences and
biomarkers is of increasing importance in diverse areas including advanced diagnostics …
biomarkers is of increasing importance in diverse areas including advanced diagnostics …
Rapid and sensitive detection of antibiotic resistance on a programmable digital microfluidic platform
The widespread dissemination of CTX-M extended spectrum β-lactamases among
Escherichia coli bacteria, both in nosocomial and community environments, is a challenge …
Escherichia coli bacteria, both in nosocomial and community environments, is a challenge …
Microfluidic-based approaches for foodborne pathogen detection
X Zhao, M Li, Y Liu - Microorganisms, 2019 - mdpi.com
Food safety is of obvious importance, but there are frequent problems caused by foodborne
pathogens that threaten the safety and health of human beings worldwide. Although the …
pathogens that threaten the safety and health of human beings worldwide. Although the …
Solid-phase recombinase polymerase amplification using an extremely low concentration of a solution primer for sensitive electrochemical detection of hepatitis B viral …
AM Ichzan, SH Hwang, H Cho, C San Fang… - Biosensors and …, 2021 - Elsevier
Recombinase polymerase amplification (RPA) is considered one of the best amplification
methods for realizing a miniaturized diagnostic instrument; however, it is notably challenging …
methods for realizing a miniaturized diagnostic instrument; however, it is notably challenging …
Solid-phase primer elongation using biotinylated dNTPs for the detection of a single nucleotide polymorphism from a fingerprick blood sample
M Jauset-Rubio, M Ortiz, CK O'Sullivan - Analytical Chemistry, 2021 - ACS Publications
Isothermal recombinase polymerase amplification-based solid-phase primer extension is
used for the optical detection of a hypertrophic cardiomyopathy associated single nucleotide …
used for the optical detection of a hypertrophic cardiomyopathy associated single nucleotide …
A highly integrated system with rapid DNA extraction, recombinase polymerase amplification, and lateral flow biosensor for on-site detection of genetically modified …
X Wang, Y Chen, X Chen, C Peng, L Wang, X Xu… - Analytica Chimica …, 2020 - Elsevier
With the large-scale planting of genetically modified (GM) crops, the development of a rapid
and convenient method for on-site monitoring GM crops is needed. In this study, a duplex …
and convenient method for on-site monitoring GM crops is needed. In this study, a duplex …
Low-cost genotyping method based on allele-specific recombinase polymerase amplification and colorimetric microarray detection
ES Yamanaka, LA Tortajada-Genaro, Á Maquieira - Microchimica Acta, 2017 - Springer
The costs of current genotyping methods limit their application to personalized therapy. The
authors describe an alternative approach for the detection of single-point-polymorphisms …
authors describe an alternative approach for the detection of single-point-polymorphisms …