This review presents the most thoroughly studied bacterial fatty acid synthetic pathway, that
of Escherichia coli and then discusses the exceptions to the E. coli pathway present in other …

The past several years have witnessed a rapidly increasing number of reports on utilizing
plasmid DNA as a vector for the introduction of genes into mammalian cells for use in both …

The internal environment of growing cells is variable and dynamic, making it difficult to
introduce reliable parts, such as promoters, for genetic engineering. Here, we applied …

Metabolic engineering studies have generally focused on manipulating enzyme levels
through either the amplification, addition, or deletion of a particular pathway. However, with …

A novel in vivo method of producing succinate has been developed. A genetically
engineered Escherichia coli strain has been constructed to meet the NADH requirement and …

AL2 and L2 are related proteins encoded by geminiviruses of the Begomovirus and
Curtovirus genera, respectively. Both are pathogenicity determinants that cause enhanced …

We have developed a method called 'gene gorging'to make precise mutations in the
Escherichia coli genome at frequencies high enough (1–15%) to allow direct identification of …

The AraC regulatory protein of the Escherichia coli ara operon has been engineered to
activate transcription in response to d-arabinose and not in response to its native effector l …

Advanced high-throughput screening methods for small molecules may have important
applications in the metabolic engineering of the biosynthetic pathways of these molecules …

It is generally known that cofactors play a major role in the production of different
fermentation products. This paper is part of a systematic study that investigates the potential …