[HTML][HTML] Unlocking the potential of optogenetics in microbial applications

M Benisch, SK Aoki, M Khammash - Current Opinion in Microbiology, 2024 - Elsevier
Optogenetics is a powerful approach that enables researchers to use light to dynamically
manipulate cellular behavior. Since the first published use of optogenetics in synthetic …

Optogenetics illuminates applications in microbial engineering

SM Hoffman, AY Tang, JL Avalos - Annual review of chemical …, 2022 - annualreviews.org
Optogenetics has been used in a variety of microbial engineering applications, such as
chemical and protein production, studies of cell physiology, and engineered microbe–host …

Enhancing bioreactor arrays for automated measurements and reactive control with ReacSight

F Bertaux, S Sosa-Carrillo, V Gross, A Fraisse… - Nature …, 2022 - nature.com
Small-scale, low-cost bioreactors provide exquisite control of environmental parameters of
microbial cultures over long durations. Their use is gaining popularity in quantitative systems …

Optogenetic strategies for the control of gene expression in yeasts

ALA Pérez, LC Piva, JPC Fulber, LMP de Moraes… - Biotechnology …, 2022 - Elsevier
Optogenetics involves the use of light to control cellular functions and has become
increasingly popular in various areas of research, especially in the precise control of gene …

The Neurospora crassa Inducible Q System Enables Simultaneous Optogenetic Amplification and Inversion in Saccharomyces cerevisiae for Bidirectional Control of …

MA Lalwani, EM Zhao, SA Wegner… - ACS Synthetic …, 2021 - ACS Publications
Bidirectional optogenetic control of yeast gene expression has great potential for
biotechnological applications. Our group has developed optogenetic inverter circuits that …

Coupling cell communication and optogenetics: implementation of a light-inducible intercellular system in yeast

V Rojas, LF Larrondo - ACS Synthetic Biology, 2022 - ACS Publications
Cell communication is a widespread mechanism in biology, allowing the transmission of
information about environmental conditions. In order to understand how cell communication …

Optogenetic control of Cdc48 for dynamic metabolic engineering in yeast

F Bezold, J Scheffer, P Wendering… - Metabolic …, 2023 - Elsevier
Dynamic metabolic engineering is a strategy to switch key metabolic pathways in microbial
cell factories from biomass generation to accumulation of target products. Here, we …

PhiReX 2.0: A Programmable and Red Light-Regulated CRISPR-dCas9 System for the Activation of Endogenous Genes in Saccharomyces cerevisiae

F Machens, G Ran, C Ruehmkorff… - ACS Synthetic …, 2023 - ACS Publications
Metabolic engineering approaches do not exclusively require fine-tuning of heterologous
genes but oftentimes also modulation or even induction of host gene expression, eg, in …

[HTML][HTML] Optogenetic tools for control of public goods in Saccharomyces cerevisiae

NM Morales, MT Patel, CJ Stewart, K Sweeney… - Msphere, 2021 - ncbi.nlm.nih.gov
Microorganisms live in dense and diverse communities, with interactions between cells
guiding community development and phenotype. The ability to perturb specific intercellular …

Modular and molecular optimization of a LOV (Light–Oxygen–Voltage)-based optogenetic switch in yeast

A Romero, V Rojas, V Delgado, F Salinas… - International Journal of …, 2021 - mdpi.com
Optogenetic switches allow light-controlled gene expression with reversible and
spatiotemporal resolution. In Saccharomyces cerevisiae, optogenetic tools hold great …