Acoustofluidic methods in cell analysis

Y Xie, H Bachman, TJ Huang - TrAC Trends in Analytical Chemistry, 2019 - Elsevier
Cellular analysis is a central concept for both biology and medicine. Over the past two
decades, acoustofluidic technologies, which marry acoustic waves with microfluidics, have …

Enzyme-free release of adhered cells from standard culture dishes using intermittent ultrasonic traveling waves

Y Kurashina, C Imashiro, M Hirano, T Kuribara… - Communications …, 2019 - nature.com
Cell detachment is essential in culturing adherent cells. Trypsinization is the most popular
detachment technique, even though it reduces viability due to the damage to the membrane …

Ultrasonic control of neurite outgrowth direction

H Maruyama, K Fujiwara, M Kumeta, D Koyama - Scientific reports, 2021 - nature.com
This study investigated a method to control neurite outgrowth direction using ultrasound
vibration. An ultrasound cell culture dish comprising a glass-bottom culture surface and a …

Enzyme‐free cell detachment mediated by resonance vibration with temperature modulation

Y Kurashina, M Hirano, C Imashiro… - Biotechnology and …, 2017 - Wiley Online Library
Cell detachment is an essential process in adherent cell culture. However, trypsinization,
which is the most popular detachment technique used in culture, damages cellular …

[HTML][HTML] Formation of large scaffold-free 3-D aggregates in a cell culture dish by ultrasound standing wave trapping

M Nakao, C Imashiro, T Kuribara, Y Kurashina… - Ultrasound in Medicine …, 2019 - Elsevier
Cellular aggregates that mimic cell–cell interactions in vitro are essential for biological
research. This study introduces a method to form large scaffold-free 3-D aggregates in a …

Theoretical modeling of tunable vibrations of three-dimensional serpentine structures for simultaneous measurement of adherent cell mass and modulus

J Zhao, W Li, X Guo, H Wang, JA Rogers, Y Huang - 2021 - Springer
Vibration-based methods can be used effectively to characterize the physical properties of
biological materials, with an increasing interest focused on the mechanics of individual …

Homogenization of initial cell distribution by secondary flow of medium improves cell culture efficiency

Y Fukuma, T Inui, C Imashiro, Y Kurashina… - PloS one, 2020 - journals.plos.org
Homogenization of the initial cell distribution is essential for effective cell development.
However, there are few previous reports on efficient cell seeding methods, even though the …

Effective and intact cell detachment from a clinically ubiquitous culture flask by combining ultrasonic wave exposure and diluted trypsin

H Tauchi, C Imashiro, T Kuribara, G Fujii… - Biotechnology and …, 2019 - Springer
Bioengineering research and applications are supported by cell culture technologies that
produce a large number of homogeneous cells. However, trypsin used in the general culture …

An effective detachment system for human induced pluripotent stem cells cultured on multilayered cultivation substrates using resonance vibrations

Y Terao, Y Kurashina, S Tohyama, Y Fukuma… - Scientific reports, 2019 - nature.com
Clinical application of human induced pluripotent stem cells (hiPSCs) has been hampered
by the lack of a practical, scalable culture system. Stacked culture plates (SCPs) have …

Method of localized removal of cells using a bolt‐clamped Langevin transducer with an ultrasonic horn

T Inui, Y Kurashina, C Imashiro… - Engineering in Life …, 2019 - Wiley Online Library
Cell isolation by eliminating undesirable cell aggregations or colonies with low activity is
essential to improve cell culture efficiency. Moreover, when creating tissues from induced …