[HTML][HTML] The E factor at 30: a passion for pollution prevention
RA Sheldon - Green Chemistry, 2023 - pubs.rsc.org
The introduction of the E Factor in 1992 focussed attention on the problem of waste
generation, defined as everything but the desired product, in chemicals manufacture and …
generation, defined as everything but the desired product, in chemicals manufacture and …
[HTML][HTML] Recent trends in biocatalysis
D Yi, T Bayer, CPS Badenhorst, S Wu… - Chemical Society …, 2021 - pubs.rsc.org
Biocatalysis has undergone revolutionary progress in the past century. Benefited by the
integration of multidisciplinary technologies, natural enzymatic reactions are constantly …
integration of multidisciplinary technologies, natural enzymatic reactions are constantly …
Power of biocatalysis for organic synthesis
CK Winkler, JH Schrittwieser, W Kroutil - ACS central science, 2021 - ACS Publications
Biocatalysis, using defined enzymes for organic transformations, has become a common
tool in organic synthesis, which is also frequently applied in industry. The generally high …
tool in organic synthesis, which is also frequently applied in industry. The generally high …
Machine learning in enzyme engineering
Enzyme engineering plays a central role in developing efficient biocatalysts for
biotechnology, biomedicine, and life sciences. Apart from classical rational design and …
biotechnology, biomedicine, and life sciences. Apart from classical rational design and …
The crucial role of methodology development in directed evolution of selective enzymes
Directed evolution of stereo‐, regio‐, and chemoselective enzymes constitutes a unique way
to generate biocatalysts for synthetically interesting transformations in organic chemistry and …
to generate biocatalysts for synthetically interesting transformations in organic chemistry and …
[HTML][HTML] The Hitchhiker's guide to biocatalysis: recent advances in the use of enzymes in organic synthesis
Enzymes are excellent catalysts that are increasingly being used in industry and academia.
This perspective is primarily aimed at synthetic organic chemists with limited experience …
This perspective is primarily aimed at synthetic organic chemists with limited experience …
Advances in ultrahigh-throughput screening for directed enzyme evolution
U Markel, KD Essani, V Besirlioglu, J Schiffels… - Chemical Society …, 2020 - pubs.rsc.org
Enzymes are versatile catalysts and their synthetic potential has been recognized for a long
time. In order to exploit their full potential, enzymes often need to be re-engineered or …
time. In order to exploit their full potential, enzymes often need to be re-engineered or …
Advances in machine learning for directed evolution
Machine learning (ML) can expedite directed evolution by allowing researchers to move
expensive experimental screens in silico. Gathering sequence-function data for training ML …
expensive experimental screens in silico. Gathering sequence-function data for training ML …
[HTML][HTML] Informed training set design enables efficient machine learning-assisted directed protein evolution
Directed evolution of proteins often involves a greedy optimization in which the mutation in
the highest-fitness variant identified in each round of single-site mutagenesis is fixed. The …
the highest-fitness variant identified in each round of single-site mutagenesis is fixed. The …
Expanding the enzyme universe with genetically encoded unnatural amino acids
I Drienovská, G Roelfes - Nature Catalysis, 2020 - nature.com
The emergence of robust methods to expand the genetic code allows incorporation of non-
canonical amino acids into the polypeptide chain of proteins, thus making it possible to …
canonical amino acids into the polypeptide chain of proteins, thus making it possible to …