Sortase‐mediated ligation (SML) has emerged as a powerful and versatile methodology for
site‐specific protein conjugation, functionalization/labeling, immobilization, and design of …

Proteins are essential biomolecules and central to biotechnological applications. In many
cases, assembly into higher-order structures is a prerequisite for protein function. Under …

The development of complex molecular scaffolds with defined folding properties represents
a central challenge in chemical research. Proteins are natural scaffolds defined by a …

Sortase enzymes are critical cysteine transpeptidases on the surface of bacteria that attach
proteins to the cell wall and are involved in the construction of bacterial pili. Due to their …

Sortase‐mediated ligation (SML) is a widely used method for peptide and protein ligation
due to ease of substrate preparation and fast enzymatic kinetics. But there are drawbacks …