Phosphorylation is one of the quickest post-translational modifications that controls
downstream signaling pathways regulating processes like cell proliferation, survival, and …

Currently, unbiased protein identification is mostly performed by directly coupling reversed-
phase liquid chromatography (RPLC) via electrospray ionization to a mass spectrometer. In …

Reversed-phase liquid chromatography is the most commonly used separation method for
shotgun proteomics. Nanoflow chromatography has emerged as the preferred …

Monodispersed silica spheres with solid core and fibrous shell were successfully
synthesized using a biphase reaction. Both the thickness and the pore size of the fibrous …

Innovative columns made with very small core–shell particles (1.0–1.4 μm) were
investigated over a wide experimental space using state-of-the-art ultra high performance …

A major challenge facing current mass spectrometry (MS)-based proteomics research is the
large concentration range displayed in biological systems, which far exceeds the dynamic …

The single‐run resolving power of current 10 μm id porous‐layer open‐tubular (PLOT)
columns has been optimized. The columns studied had a poly (styrene‐co‐divinylbenzene) …

LC-MS is an important tool for metabolomics due its high sensitivity and broad metabolite
coverage. The goal of improving resolution and decreasing analysis time in HPLC has led to …

Expanding the sequencing depth of the peptides with a statistically significant quantitative
change derived from a biological stimulation is critical. Here we demonstrate that …

Abstract In this work, 1.9 μm reversed-phase packing materials with superficially porous
structure were prepared to achieve the rapid and high efficient separation of peptides and …