Genetic and biochemical analyses of the secretory pathway have produced a detailed
picture of the molecular mechanisms involved in selective cargo transport between …

N-linked oligosaccharides arise when blocks of 14 sugars are added cotranslationally to
newly synthesized polypeptides in the endoplasmic reticulum (ER). These glycans are then …

Important Ca2+ signals in the cytosol and organelles are often extremely localized and hard
to measure. To overcome this problem we have constructed new fluorescent indicators for …

Dilated cardiomyopathy is a severe pathology of the heart with poorly understood etiology.
Disruption of the gene encoding the negative immunoregulatory receptor PD-1 in BALB/c …

STING is essential for control of infections and for tumor immunosurveillance, but it can also
drive pathological inflammation. STING resides on the endoplasmic reticulum (ER) and …

In vitro synthesis of endoplasmic reticulum-derived transpofl vesicles has been reconstituted
with washed membranes and three soluble proteins (Sarl p, Secl3p complex, and Sec23p …

Recent advances have uncovered the general protein apparatus used by all eukaryotes for
intracellular transport, including secretion and endocytosis, and for triggered exocytosis of …

Newly synthesized proteins that leave the endoplasmic reticulum (ER) are funnelled through
the Golgi complex before being sorted for transport to their different final destinations …

Dynamin is the mammalian homologue to the Drosophila shibire gene product. Mutations in
this 100-kD GTPase cause a pleiotropic defect in endocytosis. To further investigate its role …

A variety of quality control mechanisms operate in the endoplasmic reticulum and in
downstream compartments of the secretory pathway to ensure the fidelity and regulation of …