The expanding field of monoclonal antibody-based pharmaceuticals has triggered
increased interest in analytical characterization of these large proteins and in understanding …

The current status of de novo sequencing of peptides by MS/MS is reviewed with focus on
collision cell MS/MS spectra. The relation between peptide structure and observed fragment …

Identification of deamidated sites in proteins is commonly used for assignment of N-
glycosylation sites. It is also important for assessing the role of deamidation in vivo …

Proteins are composed exclusively of l-amino acids. Among elderly individuals, however, d-
aspartic acid (d-Asp) residues have been found in eye lens and brain, as well as in other …

We find that peptides containing-Asn-Gly-sequences typically show∼ 70− 80% degree of
deamidation after standard overnight (∼ 12 h) tryptic digestion at 37° C. This emphasizes …

Deamidation of asparaginyl and isomerization of aspartyl residues in proteins proceed
through a succinimide intermediate producing a mixture of aspartyl and isoaspartyl residues …

A ubiquitous yet underappreciated protein post-translational modification, isoaspartic acid
(isoAsp, isoD, or β-Asp), generated via the deamidation of asparagine or isomerization of …

Protein deamidation has been proposed to represent a “molecular clock” that progressively
disrupts protein structure and function in human degenerative diseases and natural aging …

Senile plaques and amyloid‐bearing vessels consisting of fibrillar amyloid β peptides (Aβ)
are characteristic neuropathological features of Alzheimer's disease (AD). Aβ undergo …

Plaques that characterize Alzheimer's disease accumulate over 20 years as a result of
decreased clearance of amyloid-β peptides. Such long-lived peptides are subjected to …