Microfluidic single‐cell omics analysis

X Xu, J Wang, L Wu, J Guo, Y Song, T Tian, W Wang… - Small, 2020 - Wiley Online Library
The commonly existing cellular heterogeneity plays a critical role in biological processes
such as embryonic development, cell differentiation, and disease progress. Single‐cell …

Dielectrophoresis: Developments and applications from 2010 to 2020

B Sarno, D Heineck, MJ Heller, SD Ibsen - Electrophoresis, 2021 - Wiley Online Library
The 20th century has seen tremendous innovation of dielectrophoresis (DEP) technologies,
with applications being developed in areas ranging from industrial processing to micro‐and …

[HTML][HTML] Hydro-Seq enables contamination-free high-throughput single-cell RNA-sequencing for circulating tumor cells

YH Cheng, YC Chen, E Lin, R Brien, S Jung… - Nature …, 2019 - nature.com
Molecular analysis of circulating tumor cells (CTCs) at single-cell resolution offers great
promise for cancer diagnostics and therapeutics from simple liquid biopsy. Recent …

Electromagnetic forces and torques: From dielectrophoresis to optical tweezers

M Riccardi, OJF Martin - Chemical Reviews, 2023 - ACS Publications
Electromagnetic forces and torques enable many key technologies, including optical
tweezers or dielectrophoresis. Interestingly, both techniques rely on the same physical …

CUT-LAMP: contamination-free loop-mediated isothermal amplification based on the CRISPR/Cas9 cleavage

Y Bao, Y Jiang, E Xiong, T Tian, Z Zhang, J Lv, Y Li… - ACS …, 2020 - ACS Publications
Loop-mediated isothermal amplification (LAMP) is a sensitive and widely used gene
amplification technique. However, high amplification efficiency and amplification products …

[HTML][HTML] Chemical analysis of single cells and organelles

K Hu, TDK Nguyen, S Rabasco, PE Oomen… - Analytical …, 2020 - ACS Publications
life science, clinical diagnostics, drug development, etc. Recent years have witnessed the
emergence of innovative analytical methods with high sensitivity and spatial-temporal …

Microfluidics-based single cell analysis: from transcriptomics to spatiotemporal multi-omics

J Cao, X Chen, S Huang, W Shi, Q Fan, Y Gong… - TrAC Trends in …, 2023 - Elsevier
Uncover cell composition, cellular spatial distribution, and cell-to-cell interactions in tissues
are significant for the understanding of physiological and pathological processes. Over the …

Microfluidic single-cell transcriptomics: moving towards multimodal and spatiotemporal omics

S Lin, Y Liu, M Zhang, X Xu, Y Chen, H Zhang, C Yang - Lab on a Chip, 2021 - pubs.rsc.org
Cells are the basic units of life with vast heterogeneity. Single-cell transcriptomics unveils
cell-to-cell gene expression variabilities, discovers novel cell types, and uncovers the critical …

Functional hydrogel for fast, precise and inhibition-free point-of-care bacteria analysis in crude food samples

X Lin, M Fang, C Yi, Y Jiang, C Zhang, X Pan, Z Luo - Biomaterials, 2022 - Elsevier
In this work, instead of performing nucleic acid amplification in the bulk solution, we report a
nanoporous hydrogel with controlled release function for rapid, precise, and inhibition-free …

Microfluidic analysis of circulating tumor cells and tumor-derived extracellular vesicles

F Tian, C Liu, L Lin, Q Chen, J Sun - TrAC Trends in Analytical Chemistry, 2019 - Elsevier
Liquid biopsy of circulating tumor cells (CTCs) and tumor-derived extracellular vesicles
(EVs) holds immense promise in early detection of cancer, monitoring efficacy of therapy …