Optical tweezers analysis of DNA–protein complexes
Cellular DNA is continuously engaged in a molecular dance with many accessory proteins.
These proteins are responsible for orchestrating the storage, maintenance, and transfer of …
These proteins are responsible for orchestrating the storage, maintenance, and transfer of …
[HTML][HTML] A primer on the oxDNA model of DNA: when to use it, how to simulate it and how to interpret the results
The oxDNA model of Deoxyribonucleic acid has been applied widely to systems in biology,
biophysics and nanotechnology. It is currently available via two independent open source …
biophysics and nanotechnology. It is currently available via two independent open source …
[HTML][HTML] Chromothripsis and kataegis induced by telomere crisis
Telomere crisis occurs during tumorigenesis when depletion of the telomere reserve leads
to frequent telomere fusions. The resulting dicentric chromosomes have been proposed to …
to frequent telomere fusions. The resulting dicentric chromosomes have been proposed to …
Structural, mechanical, and thermodynamic properties of a coarse-grained DNA model
We explore in detail the structural, mechanical, and thermodynamic properties of a coarse-
grained model of DNA similar to that recently introduced in a study of DNA nanotweezers …
grained model of DNA similar to that recently introduced in a study of DNA nanotweezers …
Acoustic force spectroscopy
Force spectroscopy has become an indispensable tool to unravel the structural and
mechanochemical properties of biomolecules. Here we extend the force spectroscopy …
mechanochemical properties of biomolecules. Here we extend the force spectroscopy …
[HTML][HTML] The impact of DNA intercalators on DNA and DNA-processing enzymes elucidated through force-dependent binding kinetics
AS Biebricher, I Heller, RFH Roijmans… - Nature …, 2015 - nature.com
DNA intercalators are widely used as fluorescent probes to visualize DNA and DNA
transactions in vivo and in vitro. It is well known that they perturb DNA structure and stability …
transactions in vivo and in vitro. It is well known that they perturb DNA structure and stability …
DNA stretching induces Cas9 off-target activity
CRISPR/Cas9 is a powerful genome-editing tool, but spurious off-target edits present a
barrier to therapeutic applications. To understand how CRISPR/Cas9 discriminates between …
barrier to therapeutic applications. To understand how CRISPR/Cas9 discriminates between …
Sliding sleeves of XRCC4–XLF bridge DNA and connect fragments of broken DNA
Non-homologous end joining (NHEJ) is the primary pathway for repairing DNA double-
strand breaks (DSBs) in mammalian cells. Such breaks are formed, for example, during …
strand breaks (DSBs) in mammalian cells. Such breaks are formed, for example, during …
Quantifying how DNA stretches, melts and changes twist under tension
In cells, DNA is constantly twisted, bent and stretched by numerous proteins mediating
genome transactions. Understanding these essential biological processes requires in-depth …
genome transactions. Understanding these essential biological processes requires in-depth …
[HTML][HTML] Replication fork activation is enabled by a single-stranded DNA gate in CMG helicase
MR Wasserman, GD Schauer, ME O'Donnell, S Liu - Cell, 2019 - cell.com
The eukaryotic replicative helicase CMG is a closed ring around double-stranded (ds) DNA
at origins yet must transition to single-stranded (ss) DNA for helicase action. CMG must also …
at origins yet must transition to single-stranded (ss) DNA for helicase action. CMG must also …