PCR inhibition in qPCR, dPCR and MPS—mechanisms and solutions
DNA analysis has seen an incredible development in terms of instrumentation, assays and
applications over the last years. Massively parallel sequencing (MPS) and digital PCR are …
applications over the last years. Massively parallel sequencing (MPS) and digital PCR are …
Current commercial dPCR platforms: Technology and market review
LL Tan, N Loganathan, S Agarwalla… - Critical Reviews in …, 2023 - Taylor & Francis
Digital polymerase chain reaction (dPCR) technology has provided a new technique for
molecular diagnostics, with superior advantages, such as higher sensitivity, precision, and …
molecular diagnostics, with superior advantages, such as higher sensitivity, precision, and …
Single-exosome-counting immunoassays for cancer diagnostics
Exosomes shed by tumor cells have been recognized as promising biomarkers for cancer
diagnostics due to their unique composition and functions. Quantification of low …
diagnostics due to their unique composition and functions. Quantification of low …
Inhibition mechanisms of hemoglobin, immunoglobulin G, and whole blood in digital and real-time PCR
M Sidstedt, J Hedman, EL Romsos, L Waitara… - Analytical and …, 2018 - Springer
Blood samples are widely used for PCR-based DNA analysis in fields such as diagnosis of
infectious diseases, cancer diagnostics, and forensic genetics. In this study, the mechanisms …
infectious diseases, cancer diagnostics, and forensic genetics. In this study, the mechanisms …
Biomimetic virus-like particles as severe acute respiratory syndrome coronavirus 2 diagnostic tools
SK Chan, P Du, C Ignacio, S Mehta, IG Newton… - ACS …, 2020 - ACS Publications
Coronavirus disease 2019 (COVID-19) is a highly transmissible disease that has affected
more than 90% of the countries worldwide. At least 17 million individuals have been …
more than 90% of the countries worldwide. At least 17 million individuals have been …
A review of environmental DNA field and laboratory protocols applied in fish ecology and environmental health
Y Xing, W Gao, Z Shen, Y Zhang, J Bai… - Frontiers in …, 2022 - frontiersin.org
Environmental DNA (eDNA) has been used in research relevant to fish ecology such as
species diversity and conservation studies, threatened and invasive species monitoring, and …
species diversity and conservation studies, threatened and invasive species monitoring, and …
A digitalized isothermal nucleic acid testing platform based on a pump-free open droplet array microfluidic chip
Digital PCR has shown great potential for quantitative nucleic acid testing (NAT), but most
existing platforms are dependent on large auxiliary equipment (eg, vacuum pump …
existing platforms are dependent on large auxiliary equipment (eg, vacuum pump …
Molybdenum disulfide sphere-based electrochemical aptasensors for protein detection
HL Shuai, X Wu, KJ Huang - Journal of Materials Chemistry B, 2017 - pubs.rsc.org
In this work, we report the development of an ultrasensitive sandwich-type electrochemical
aptasensor for protein detection. The aptasensor is fabricated by using nitrogen-doped …
aptasensor for protein detection. The aptasensor is fabricated by using nitrogen-doped …
Comparison of protocols and RNA carriers for plasma miRNA isolation. Unraveling RNA carrier influence on miRNA isolation
LA Ramon-Nunez, L Martos, A Fernandez-Pardo, J Oto… - Plos one, 2017 - journals.plos.org
microRNAs are promising biomarkers in biological fluids in several diseases. Different
plasma RNA isolation protocols and carriers are available, but their efficiencies have been …
plasma RNA isolation protocols and carriers are available, but their efficiencies have been …
Adaptive filtering framework to remove nonspecific and low-efficiency reactions in multiplex digital PCR based on sigmoidal trends
Real-time digital polymerase chain reaction (qdPCR) coupled with machine learning (ML)
methods has shown the potential to unlock scientific breakthroughs, particularly in the field …
methods has shown the potential to unlock scientific breakthroughs, particularly in the field …