PCR inhibition in qPCR, dPCR and MPS—mechanisms and solutions

M Sidstedt, P Rådström, J Hedman - Analytical and bioanalytical chemistry, 2020 - Springer
DNA analysis has seen an incredible development in terms of instrumentation, assays and
applications over the last years. Massively parallel sequencing (MPS) and digital PCR are …

Current commercial dPCR platforms: Technology and market review

LL Tan, N Loganathan, S Agarwalla… - Critical Reviews in …, 2023 - Taylor & Francis
Digital polymerase chain reaction (dPCR) technology has provided a new technique for
molecular diagnostics, with superior advantages, such as higher sensitivity, precision, and …

Single-exosome-counting immunoassays for cancer diagnostics

C Liu, X Xu, B Li, B Situ, W Pan, Y Hu, T An, S Yao… - Nano …, 2018 - ACS Publications
Exosomes shed by tumor cells have been recognized as promising biomarkers for cancer
diagnostics due to their unique composition and functions. Quantification of low …

Inhibition mechanisms of hemoglobin, immunoglobulin G, and whole blood in digital and real-time PCR

M Sidstedt, J Hedman, EL Romsos, L Waitara… - Analytical and …, 2018 - Springer
Blood samples are widely used for PCR-based DNA analysis in fields such as diagnosis of
infectious diseases, cancer diagnostics, and forensic genetics. In this study, the mechanisms …

Biomimetic virus-like particles as severe acute respiratory syndrome coronavirus 2 diagnostic tools

SK Chan, P Du, C Ignacio, S Mehta, IG Newton… - ACS …, 2020 - ACS Publications
Coronavirus disease 2019 (COVID-19) is a highly transmissible disease that has affected
more than 90% of the countries worldwide. At least 17 million individuals have been …

A review of environmental DNA field and laboratory protocols applied in fish ecology and environmental health

Y Xing, W Gao, Z Shen, Y Zhang, J Bai… - Frontiers in …, 2022 - frontiersin.org
Environmental DNA (eDNA) has been used in research relevant to fish ecology such as
species diversity and conservation studies, threatened and invasive species monitoring, and …

A digitalized isothermal nucleic acid testing platform based on a pump-free open droplet array microfluidic chip

P Mao, L Cao, Z Li, M You, B Gao, X Xie, Z Xue, P Peng… - Analyst, 2021 - pubs.rsc.org
Digital PCR has shown great potential for quantitative nucleic acid testing (NAT), but most
existing platforms are dependent on large auxiliary equipment (eg, vacuum pump …

Molybdenum disulfide sphere-based electrochemical aptasensors for protein detection

HL Shuai, X Wu, KJ Huang - Journal of Materials Chemistry B, 2017 - pubs.rsc.org
In this work, we report the development of an ultrasensitive sandwich-type electrochemical
aptasensor for protein detection. The aptasensor is fabricated by using nitrogen-doped …

Comparison of protocols and RNA carriers for plasma miRNA isolation. Unraveling RNA carrier influence on miRNA isolation

LA Ramon-Nunez, L Martos, A Fernandez-Pardo, J Oto… - Plos one, 2017 - journals.plos.org
microRNAs are promising biomarkers in biological fluids in several diseases. Different
plasma RNA isolation protocols and carriers are available, but their efficiencies have been …

Adaptive filtering framework to remove nonspecific and low-efficiency reactions in multiplex digital PCR based on sigmoidal trends

L Miglietta, K Xu, P Chhaya, L Kreitmann… - Analytical …, 2022 - ACS Publications
Real-time digital polymerase chain reaction (qdPCR) coupled with machine learning (ML)
methods has shown the potential to unlock scientific breakthroughs, particularly in the field …