Synthetic biology concerns the engineering of man‐made living biomachines from
standardized components that can perform predefined functions in a (self‐) controlled …

Allosteric transition, defined as conformational changes induced by ligand binding, is one of
the fundamental properties of proteins. Allostery has been observed and characterized in …

Allosteric regulation of protein function is widespread in biology, but is challenging for de
novo protein design as it requires the explicit design of multiple states with comparable free …

Genetic regulatory proteins inducible by small molecules are useful synthetic biology tools
as sensors and switches. Bacterial allosteric transcription factors (aTFs) are a major class of …

Praise for the first edition:… superb, beautifully written and organized work that takes an
engineering approach to systems biology. Alon provides nicely written appendices to …

Engineering cells to produce valuable metabolic products is hindered by the slow and
laborious methods available for evaluating product concentration. Consequently, many …

Recent efforts in biological engineering have made detection of nucleic acids in samples
more rapid, inexpensive and sensitive using CRISPR-based approaches. We expand one of …

The aryl hydrocarbon receptor (AHR) belongs to the PAS (PER-ARNT-SIM) family
transcription factors and mediates broad responses to numerous environmental pollutants …

Monitoring cellular behavior and eventually properly adapting cellular processes is key to
handle the enormous complexity of today's metabolic engineering questions. Hence …

Allosteric regulation, the most direct and efficient way of regulating protein function, is
induced by the binding of a ligand at one site that is topographically distinct from an …