Enzymes can be engineered at the level of their amino acid sequences to optimize key
properties such as expression, stability, substrate range, and catalytic efficiency─ or even to …

The development of artificial metalloenzymes (ArMs) aims to expand the capabilities of
enzymatic catalysis, most notably towards new reaction mechanisms. Frequently, ArMs …

Designing protein sequences towards desired properties is a fundamental goal of protein
engineering, with applications in drug discovery and enzymatic engineering. Machine …

Protein engineering often targets binding pockets or active sites which are enriched in
epistasis—nonadditive interactions between amino acid substitutions—and where the …

Biocatalysis is a key technology in the pharmaceutical industry as it can provide efficient and
sustainable catalytic routes toward active pharmaceutical ingredients (APIs). The …

Engineering proteins and enzymes with the desired functionality has broad applications in
molecular biology, biotechnology, biomedical sciences, health, and medicine. The vastness …

Gas vesicles (GVs) are gas-filled protein nanostructures employed by several species of
bacteria and archaea as flotation devices to enable access to optimal light and nutrients …

Tailored enzymes are crucial for the transition to a sustainable bioeconomy. However,
enzyme engineering is laborious and failure-prone due to its reliance on serendipity. The …

Sequence-function data provides valuable information about the protein functional
landscape but is rarely obtained during directed evolution campaigns. Here, we present …

Highlights•Systems metabolic engineering has enabled efficient production of natural
products.•Systems-level engineering of metabolism has increased pathway efficiencies.•Cell …