Automation is playing an increasingly significant role in synthetic biology. Groundbreaking
technologies, developed over the past 20 years, have enormously accelerated the …

Comprehensive analysis of gene function requires the detailed examination of mutant
alleles. In Arabidopsis thaliana, large collections of sequence‐indexed insertion and …

The foundational adenine base editors (for example, ABE7. 10) enable programmable A• T
to G• C point mutations but editing efficiencies can be low at challenging loci in primary …

Current approaches for the production of high-value compounds in microorganisms mostly
use the cytosol as a general reaction vessel. However, competing pathways and metabolic …

Most land plant genomes carry genes that encode RPW 8‐NLR Resistance (R) proteins.
Angiosperms carry two RPW 8‐NLR subclasses: ADR 1 and NRG 1. ADR 1s act as …

We describe an isothermal, single-reaction method for assembling multiple overlapping
DNA molecules by the concerted action of a 5′ exonuclease, a DNA polymerase and a …

The ginkgo tree (Ginkgo biloba) is considered a living fossil due to its 200 million year's
history under morphological stasis. Its resilience is partly attributed to its unique set of …

We analyze the structure of the cytoplasm by performing single-molecule displacement
mapping on a diverse set of native cytoplasmic proteins in exponentially growing …

Plant synthetic biology aims to apply engineering principles to plant genetic design. One
strategic requirement of plant synthetic biology is the adoption of common standardized …

In vivo homologous recombination holds the potential for optimal molecular cloning,
however, current strategies require specialised bacterial strains or laborious protocols. Here …