The life of eukaryotic cells requires the transport of lipids between membranes, which are
separated by the aqueous environment of the cytosol. Vesicle-mediated traffic along the …

Most life scientists would agree that understanding how cellular processes work requires
structural knowledge about the macromolecules involved. For example, deciphering the …

Cryo-electron tomography (cryo-ET) allows for label-free high-resolution imaging of
macromolecular assemblies in their native cellular context. However, the localization of …

In the last decade, molecular dynamics (MD) simulations have become an essential tool to
investigate the molecular properties of membrane trafficking processes, often in conjunction …

Most mitochondrial proteins are synthesized on cytosolic ribosomes and imported into
mitochondria in a post-translational reaction. Mitochondrial precursor proteins which use the …

Biology spans a continuum of length and time scales. Individual experimental methods only
glimpse discrete pieces of this spectrum but can be combined to construct a more holistic …

The view of organelles and how they operate together has changed dramatically over the
last two decades. The textbook view of organelles was that they operated largely …

Cryo-electron tomography (CryoET) images the cellular environment in situ, without labels,
and with fully preserved context. However, the complex cellular environment and the low …

Membrane contact sites (MCSs) are areas of close proximity between organelles, implicated
in transport of small molecules and in organelle biogenesis. Lipid transfer proteins at MCSs …

Electron microscopy (EM), in its various flavors, has significantly contributed to our
understanding of lipid droplets (LD) as central organelles in cellular metabolism. For …