The global demand for data storage is currently outpacing the world's storage capabilities.
DNA, the carrier of natural genetic information, offers a stable, resource-and energy-efficient …

The assembly of DNA from small fragments into large constructs has seen significant recent
development, becoming a pivotal technology in the ability to implement the vision of …

Plant Synthetic Biology requires robust and efficient methods for assembling multigene
constructs. Golden Gate cloning provides a precision module-based cloning technique for …

The field of synthetic biology promises to revolutionize biotechnology through the design of
organisms with novel phenotypes useful for medicine, agriculture and industry. However, a …

Efforts to improve the production of a compound of interest in Saccharomyces cerevisiae
have mainly involved engineering or overexpression of cytoplasmic enzymes. We show that …

In vivo homologous recombination holds the potential for optimal molecular cloning,
however, current strategies require specialised bacterial strains or laborious protocols. Here …

Basic research has provided a much better understanding of the genetic networks and
regulatory hierarchies in plants. To meet the challenges of agriculture, we must be able to …

Assembly cloning is increasingly replacing conventional restriction enzyme and DNA-ligase-
dependent cloning methods for reasons of efficiency and performance. Here, we describe …

Plant natural products have been extensively exploited in food, medicine, flavor, cosmetic,
renewable fuel, and other industrial sectors. Synthetic biology has recently emerged as a …

Molecular cloning is utilized in nearly every facet of biological and medical research. We
have developed a method, termed Hot Fusion, to efficiently clone one or multiple DNA …