Yeast expression systems: overview and recent advances

R Baghban, S Farajnia, M Rajabibazl, Y Ghasemi… - Molecular …, 2019 - Springer
Yeasts are outstanding hosts for the production of functional recombinant proteins with
industrial or medical applications. Great attention has been emerged on yeast due to the …

The yeast deletion collection: a decade of functional genomics

G Giaever, C Nislow - Genetics, 2014 - academic.oup.com
The yeast deletion collections comprise> 21,000 mutant strains that carry precise start-to-
stop deletions of∼ 6000 open reading frames. This collection includes heterozygous and …

[HTML][HTML] Cellular control of viscosity counters changes in temperature and energy availability

LB Persson, VS Ambati, O Brandman - Cell, 2020 - cell.com
Cellular functioning requires the orchestration of thousands of molecular interactions in time
and space. Yet most molecules in a cell move by diffusion, which is sensitive to external …

[HTML][HTML] High-resolution lineage tracking reveals travelling wave of adaptation in laboratory yeast

AN Nguyen Ba, I Cvijović, JI Rojas Echenique… - Nature, 2019 - nature.com
In rapidly adapting asexual populations, including many microbial pathogens and viruses,
numerous mutant lineages often compete for dominance within the population,,,–. These …

[HTML][HTML] A versatile ultra-high performance LC-MS method for lipid profiling

OL Knittelfelder, BP Weberhofer, TO Eichmann… - … of Chromatography B, 2014 - Elsevier
A new UPLC-based untargeted lipidomic approach using a qTOF hybrid mass spectrometer
is introduced. The applied binary gradient enables separations of lipid species including …

High and stable ATP levels prevent aberrant intracellular protein aggregation in yeast

M Takaine, H Imamura, S Yoshida - Elife, 2022 - elifesciences.org
Adenosine triphosphate (ATP) at millimolar levels has recently been implicated in the
solubilization of cellular proteins. However, the significance of this high ATP level under …

Engineered protein degradation of farnesyl pyrophosphate synthase is an effective regulatory mechanism to increase monoterpene production in Saccharomyces …

B Peng, LK Nielsen, SC Kampranis, CE Vickers - Metabolic engineering, 2018 - Elsevier
Monoterpene production in Saccharomyces cerevisae requires the introduction of
heterologous monoterpene synthases (MTSs). The endogenous farnesyl pyrosphosphate …

Vitamin requirements and biosynthesis in Saccharomyces cerevisiae

T Perli, AK Wronska, RA Ortiz‐Merino, JT Pronk… - Yeast, 2020 - Wiley Online Library
Chemically defined media for yeast cultivation (CDMY) were developed to support fast
growth, experimental reproducibility, and quantitative analysis of growth rates and biomass …

Optimization of lipid production with a genome-scale model of Yarrowia lipolytica

M Kavšček, G Bhutada, T Madl, K Natter - BMC systems biology, 2015 - Springer
Background Yarrowia lipolytica is a non-conventional yeast that is extensively investigated
for its ability to excrete citrate or to accumulate large amounts of storage lipids, which is of …

Peroxisome function relies on organelle-associated mRNA translation

N Dahan, YS Bykov, EA Boydston, A Fadel, Z Gazi… - Science …, 2022 - science.org
Crucial metabolic functions of peroxisomes rely on a variety of peroxisomal membrane
proteins (PMPs). While mRNA transcripts of PMPs were shown to be colocalized with …