High-throughput and single-cell T cell receptor sequencing technologies

JA Pai, AT Satpathy - Nature methods, 2021 - nature.com
T cells express T cell receptors (TCRs) composed of somatically recombined TCRα and
TCRβ chains, which mediate recognition of major histocompatibility complex (MHC)–antigen …

Understanding the drivers of MHC restriction of T cell receptors

NL La Gruta, S Gras, SR Daley, PG Thomas… - Nature Reviews …, 2018 - nature.com
T cell discrimination of self and non-self is predicated on αβ T cell receptor (TCR) co-
recognition of peptides presented by MHC molecules. Over the past 20 years, structurally …

Overview of methodologies for T-cell receptor repertoire analysis

E Rosati, CM Dowds, E Liaskou, EKK Henriksen… - BMC …, 2017 - Springer
Background The T-cell receptor (TCR), located on the surface of T cells, is responsible for
the recognition of the antigen-major histocompatibility complex, leading to the initiation of an …

T cell fate and clonality inference from single-cell transcriptomes

MJT Stubbington, T Lönnberg, V Proserpio, S Clare… - Nature …, 2016 - nature.com
We developed TraCeR, a computational method to reconstruct full-length, paired T cell
receptor (TCR) sequences from T lymphocyte single-cell RNA sequence data. TraCeR links …

[HTML][HTML] Library preparation for next generation sequencing: A review of automation strategies

JF Hess, TA Kohl, M Kotrová, K Rönsch… - Biotechnology …, 2020 - Elsevier
Next generation sequencing is in the process of evolving from a technology used for
research purposes to one which is applied in clinical diagnostics. Recently introduced high …

[HTML][HTML] T-cell repertoire analysis and metrics of diversity and clonality

J Chiffelle, R Genolet, MAS Perez, G Coukos… - Current opinion in …, 2020 - Elsevier
Highlights•Immune fitness in health and disease depends notably on the diversity of cellular
repertoires.•Single-cell sequencing is fostering the understanding of clonal diversity of T-cell …

In-depth determination and analysis of the human paired heavy-and light-chain antibody repertoire

BJ DeKosky, T Kojima, A Rodin, W Charab… - Nature medicine, 2015 - nature.com
High-throughput immune repertoire sequencing has emerged as a critical step in the
understanding of adaptive responses following infection or vaccination or in autoimmunity …

Single cell T cell receptor sequencing: techniques and future challenges

M De Simone, G Rossetti, M Pagani - Frontiers in Immunology, 2018 - frontiersin.org
The peculiarity of T cell is their ability to recognize an infinite range of self and foreign
antigens. This ability is achieved during thymic development through a complex molecular …

Functional TCR T cell screening using single-cell droplet microfluidics

AI Segaliny, G Li, L Kong, C Ren, X Chen, JK Wang… - Lab on a Chip, 2018 - pubs.rsc.org
Adoptive T cell transfer, in particular TCR T cell therapy, holds great promise for cancer
immunotherapy with encouraging clinical results. However, finding the right TCR T cell clone …

Massively parallel sequencing of single cells by epicPCR links functional genes with phylogenetic markers

SJ Spencer, MV Tamminen, SP Preheim… - The ISME …, 2016 - academic.oup.com
Many microbial communities are characterized by high genetic diversity. 16S ribosomal
RNA sequencing can determine community members, and metagenomics can determine …