High-throughput screening for efficient microbial biotechnology
Highlights•High-throughput screening (HTS) facilitates selection of the best performing
strain.•Biosensor-enabled HTS obviates the need for sophisticated analytical …
strain.•Biosensor-enabled HTS obviates the need for sophisticated analytical …
Synthetic biology approaches to improve biocatalyst identification in metagenomic library screening
ME Guazzaroni, R Silva‐Rocha… - Microbial …, 2015 - Wiley Online Library
There is a growing demand for enzymes with improved catalytic performance or tolerance to
process‐specific parameters, and biotechnology plays a crucial role in the development of …
process‐specific parameters, and biotechnology plays a crucial role in the development of …
Microbial taxonomy in the era of OMICS: application of DNA sequences, computational tools and techniques
The current prokaryotic taxonomy classifies phenotypically and genotypically diverse
microorganisms using a polyphasic approach. With advances in the next-generation …
microorganisms using a polyphasic approach. With advances in the next-generation …
Comparative genomic analysis of nine Sphingobium strains: insights into their evolution and hexachlorocyclohexane (HCH) degradation pathways
Background Sphingobium spp. are efficient degraders of a wide range of chlorinated and
aromatic hydrocarbons. In particular, strains which harbour the lin pathway genes mediating …
aromatic hydrocarbons. In particular, strains which harbour the lin pathway genes mediating …
Restoring HCHs polluted land as one of the priority activities during the UN-International Decade on Ecosystem Restoration (2021–2030): A call for global action
Abstract The United Nations General Assembly has recently declared 2021–2030 as the
'International Decade on Ecosystem Restoration'for facilitating the restoration of degraded …
'International Decade on Ecosystem Restoration'for facilitating the restoration of degraded …
Haloalkane dehalogenases from marine organisms
Haloalkane dehalogenases degrade halogenated compounds to corresponding alcohols by
a hydrolytic mechanism. These enzymes are being intensively investigated as model …
a hydrolytic mechanism. These enzymes are being intensively investigated as model …
Stereoselective accumulations of hexachlorocyclohexanes (HCHs) are correlated with Sphingomonas spp. in agricultural soils across China
The wide usage of hexachlorocyclohexanes (HCHs) as pesticides has caused soil pollution
and adverse health effects through direct contact or bioaccumulation in the food chain. This …
and adverse health effects through direct contact or bioaccumulation in the food chain. This …
Functional screening of a soil metagenome for DNA endonucleases by acquired resistance to bacteriophage infection
Endonucleases play a crucial role as reagents in laboratory research and diagnostics. Here,
metagenomics was used to functionally screen a fosmid library for endonucleases. A fosmid …
metagenomics was used to functionally screen a fosmid library for endonucleases. A fosmid …
A high-throughput screening assay for the directed evolution-guided discovery of halohydrin dehalogenase mutants for epoxide ring-opening reaction
Halohydrin dehalogenases (HHDHs) are valuable biocatalysts involved in the synthesis of β-
substituted alcohols via their nucleophile-mediated ring-opening activity. To use directed …
substituted alcohols via their nucleophile-mediated ring-opening activity. To use directed …
A paper-based whole-cell screening assay for directed evolution-driven enzyme engineering
Directed evolution has become an important method to unleash the latent potential of
enzymes to make them uniquely suited for human purposes. However, the need for a large …
enzymes to make them uniquely suited for human purposes. However, the need for a large …