Actin plays many well-known roles in cells, and understanding any specific role is often
confounded by the overlap of multiple actin-based structures in space and time. Here, we …

Many fundamental cellular processes such as division, polarization, endocytosis, and
motility require the assembly, maintenance, and disassembly of filamentous actin (F-actin) …

Asymmetric cell division, which includes cell polarization and cytokinesis, is essential for
generating cell diversity during development. The budding yeast Saccharomyces cerevisiae …

Controlling the quantity and size of organelles through competition for a limited supply of
components is quickly emerging as an important cellular regulatory mechanism [1]. Cells …

Animal cell shape is controlled primarily by the actomyosin cortex, a thin cytoskeletal
network that lies directly beneath the plasma membrane. The cortex regulates cell …

Cell division facilitated by a contractile ring is an almost universal feature across all
branches of cellular life, with the notable exception of higher plants. In all organisms that use …

Mitochondrial dynamics, including fusion, fission and translocation, are crucial to cellular
homeostasis, with roles in cellular polarity, stress response and apoptosis. Mitochondrial …

Cytokinesis in animals, fungi, and amoebas depends on the constriction of a contractile ring
built from a common set of conserved proteins. Many fundamental questions remain about …

Anillins and Mid1 are scaffold proteins that play key roles in anchorage of the contractile ring
at the cell equator during cytokinesis in animals and fungi, respectively. Here, we report …

In recent years, quantification of absolute protein numbers in cellular structures using
fluorescence microscopy has become a reality. Two popular methods are available to a …