Single-cell microfluidic impedance cytometry: From raw signals to cell phenotypes using data analytics

C Honrado, P Bisegna, NS Swami, F Caselli - Lab on a Chip, 2021 - pubs.rsc.org
The biophysical analysis of single-cells by microfluidic impedance cytometry is emerging as
a label-free and high-throughput means to stratify the heterogeneity of cellular systems …

Positional dependence of particles and cells in microfluidic electrical impedance flow cytometry: Origin, challenges and opportunities

H Daguerre, M Solsona, J Cottet, M Gauthier… - Lab on a Chip, 2020 - pubs.rsc.org
Microfluidic electrical impedance flow cytometry is now a well-known and established
method for single-cell analysis. Given the richness of the information provided by impedance …

Neural network-enhanced real-time impedance flow cytometry for single-cell intrinsic characterization

Y Feng, Z Cheng, H Chai, W He, L Huang, W Wang - Lab on a Chip, 2022 - pubs.rsc.org
Single-cell impedance flow cytometry (IFC) is emerging as a label-free and non-invasive
method for characterizing the electrical properties and revealing sample heterogeneity. At …

Microfluidic impedance cytometry for single-cell sensing: Review on electrode configurations

S Zhu, X Zhang, Z Zhou, Y Han, N Xiang, Z Ni - Talanta, 2021 - Elsevier
Single-cell analysis has gained considerable attention for disease diagnosis, drug
screening, and differentiation monitoring. Compared to the well-established flow cytometry …

Modified red blood cells as multimodal standards for benchmarking single-cell cytometry and separation based on electrical physiology

A Salahi, C Honrado, A Rane, F Caselli… - Analytical …, 2022 - ACS Publications
Biophysical cellular information at single-cell sensitivity is becoming increasingly important
within analytical and separation platforms that associate the cell phenotype with markers of …

A neural network approach for real-time particle/cell characterization in microfluidic impedance cytometry

C Honrado, JS McGrath, R Reale, P Bisegna… - Analytical and …, 2020 - Springer
Microfluidic applications such as active particle sorting or selective enrichment require
particle classification techniques that are capable of working in real time. In this paper, we …

Signal-based methods in dielectrophoresis for cell and particle separation

M Farasat, E Aalaei, S Kheirati Ronizi, A Bakhshi… - Biosensors, 2022 - mdpi.com
Separation and detection of cells and particles in a suspension are essential for various
applications, including biomedical investigations and clinical diagnostics. Microfluidics …

Fine-tuning the characteristic of the applied potential to improve AC-iEK separations of microparticles

NNN Ahamed, CA Mendiola-Escobedo… - Analytical …, 2023 - ACS Publications
There is an immediate need for the development of rapid and reliable methods for
microparticle and cell assessments, and electrokinetic (EK) phenomena can be exploited to …

Dielectrophoresis in cell characterization

EA Henslee - Electrophoresis, 2020 - Wiley Online Library
Many cellular functions are affected by and thus can be characterized by a cell's
electrophysiology. This has also been found to correspond to other biophysical parameters …

Recent advances in electrical impedance sensing technology for single-cell analysis

Z Zhang, X Huang, K Liu, T Lan, Z Wang, Z Zhu - Biosensors, 2021 - mdpi.com
Cellular heterogeneity is of significance in cell-based assays for life science, biomedicine
and clinical diagnostics. Electrical impedance sensing technology has become a powerful …