Targeted covalent inhibitors (TCIs) are designed to bind poorly conserved amino acids by
means of reactive groups, the so-called warheads. Currently, targeting noncatalytic cysteine …

The native chemical ligation reaction (NCL) involves reacting a C-terminal peptide thioester
with an N-terminal cysteinyl peptide to produce a native peptide bond between the two …

Charged and polar groups, through forming ion pairs, hydrogen bonds, and other less
specific electrostatic interactions, impart important properties to proteins. Modulation of the …

Isobaric stable isotope labeling using, for example, tandem mass tags (TMTs) is increasingly
being applied for large-scale proteomic studies. Experiments focusing on proteoform …

Background Accurate estimation of the isoelectric point (pI) based on the amino acid
sequence is useful for many analytical biochemistry and proteomics techniques such as 2-D …

The accuracy of atomistic biomolecular modeling and simulation studies depend on the
accuracy of the input structures. Preparing these structures for an atomistic modeling task …

Proteome-pI is an online database containing information about predicted isoelectric points
for 5029 proteomes calculated using 18 methods. The isoelectric point, the pH at which a …

Coacervates droplets have long been considered as potential protocells to mimic living
cells. However, these droplets lack a membrane and are prone to coalescence, limiting their …

The isoelectric point is the pH at which a particular molecule is electrically neutral due to the
equilibrium of positive and negative charges. In proteins and peptides, this depends on the …

Using complementary approaches of potentiometry and NMR spectroscopy, we have
determined that the equilibrium acid dissociation constant (pKa value) of the arginine …