Advances and trends in microbial production of polyhydroxyalkanoates and their building blocks

Q Gao, H Yang, C Wang, XY Xie, KX Liu… - … in Bioengineering and …, 2022 - frontiersin.org
With the rapid development of synthetic biology, a variety of biopolymers can be obtained by
recombinant microorganisms. Polyhydroxyalkanoates (PHA) is one of the most popular one …

Polyhydroxyalkanoate synthase (PhaC): The key enzyme for biopolyester synthesis

SZ Neoh, MF Chek, HT Tan, JA Linares-Pastén… - Current Research in …, 2022 - Elsevier
Polyhydroxyalkanoates (PHAs) are considered good candidates in replacing commercial
petrochemical plastics in certain applications like single-use packaging since they are …

Production of PHA copolymers consisting of 3-hydroxybutyrate and 3-hydroxyhexanoate (PHBHHx) by recombinant Halomonas bluephagenesis

H Wang, JW Ye, X Chen, Y Yuan, J Shi, X Liu… - Chemical Engineering …, 2023 - Elsevier
Abstract Microbial synthesized poly (3-hydroxybutyrate-co-3-hydroxyhexanoate), namely
PHBHHx, with different 3HHx monomer ratios have shown biocompatibility, biodegradability …

Biotechnological Conversion of Grape Pomace to Poly(3-hydroxybutyrate) by Moderately Thermophilic Bacterium Tepidimonas taiwanensis

X Kourilova, I Pernicova, M Vidlakova, R Krejcirik… - Bioengineering, 2021 - mdpi.com
Polyhydroxyalkanoates (PHA) are microbial polyesters that have recently come to the
forefront of interest due to their biodegradability and production from renewable sources. A …

Directed evolution of sequence-regulating polyhydroxyalkanoate synthase to synthesize a Medium-Chain-Length–Short-Chain-Length (MCL–SCL) block copolymer

HT Phan, Y Hosoe, M Guex, M Tomoi… - …, 2022 - ACS Publications
Sequence-regulating polyhydroxyalkanoate synthase PhaCAR is a chimeric enzyme
comprising PhaCs from Aeromonas caviae and Ralstonia eutropha (Cupriavidus necator). It …

Toward the production of block copolymers in microbial cells: achievements and perspectives

K Matsumoto - Applied Microbiology and Biotechnology, 2024 - Springer
The microbial production of polyhydroxyalkanoate (PHA) block copolymers has attracted
research interests because they can be expected to exhibit excellent physical properties …

Characterization of an (R)-specific enoyl-CoA hydratase from Streptomyces sp. strain CFMR 7: A metabolic tool for enhancing the production of poly (3 …

HT Tan, MF Chek, Y Miyahara, SY Kim, T Tsuge… - Journal of bioscience …, 2022 - Elsevier
Poly [(R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate][P (3HB-co-3HHx)] has a high
potential to serve as a commercial bioplastic due to its biodegradability, thermoplastic and …

Biosynthesis of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) in metabolically recombinant Escherichia coli

C Miao, D Meng, Y Liu, F Wang, L Chen… - International Journal of …, 2021 - Elsevier
In this study, a phaC R gene encoding PHA synthase was identified in Rhodoligotrophos
defluvii which was adjacent to β-ketothiolase encoded by phaA R gene and acetoacetyl …

Engineering of the Long-Main-Chain Monomer-Incorporating Polyhydroxyalkanoate Synthase PhaCAR for the Biosynthesis of Poly[(R)-3-hydroxybutyrate-co-6 …

Y Hozumi, S Hachisuka, H Tomita, H Kikukawa… - …, 2024 - ACS Publications
Polyhydroxyalkanoate (PHA) synthases (PhaCs) are useful and versatile tools for the
production of aliphatic polyesters. Here, the chimeric PHA synthase PhaCAR was …

N-terminal truncation of PhaCBP-M-CPF4 and its effect on PHA production

SZ Neoh, HT Tan, C Trakunjae, MF Chek… - Microbial Cell …, 2024 - Springer
Background Among the polyhydroxyalkanoate (PHA), poly [(R)-3-hydroxybutyrate-co-(R)-3-
hydroxyhexanoate][P (3HB-co-3HHx)] is reported to closely resemble polypropylene and …