Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …

Eukaryotic cells deploy overlapping repair pathways to resolve DNA damage.
Advancements in genome editing take advantage of these pathways to produce permanent …

Genome editing has therapeutic potential for treating genetic diseases and cancer.
However, the currently most practicable approaches rely on the generation of DNA double …

Prime editing (PE) is a powerful genome engineering approach that enables the introduction
of base substitutions, insertions and deletions into any given genomic locus. However, the …

Abstract Homology-directed repair (HDR), a method for repair of DNA double-stranded
breaks can be leveraged for the precise introduction of mutations supplied by synthetic DNA …

At present, the idea of genome modification has revolutionized the modern therapeutic
research era. Genome modification studies have traveled a long way from gene …

Most methods for the detection of nucleic acids require many reagents and expensive and
bulky instrumentation. Here, we report the development and testing of a graphene-based …

Clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9
(Cas9) is a precise genome manipulating technology that can be programmed to induce …

A robust genome editing pipeline is critical to the vitality of a modern genetic laboratory.
Previous studies have shown that Cas9 ribonucleoprotein (RNP)-based editing can be …

CRISPR-Cas genome editing induces targeted DNA damage but can also affect off-target
sites. Current off-target discovery methods work using purified DNA or specific cellular …