Cre recombinase

GD Van Duyne - Mobile DNA III, 2015 - Wiley Online Library
The use of Cre recombinase to carry out conditional mutagenesis of transgenes and insert
DNA cassettes into eukaryotic chromosomes is widespread (1–9). Indeed, a PubMed search …

Bacteriophage-encoded bacterial virulence factors and phage–pathogenicity island interactions

EF Boyd - Advances in virus research, 2012 - Elsevier
The role of bacteriophages as natural vectors for some of the most potent bacterial toxins is
well recognized and includes classical type I membrane-acting superantigens, type II pore …

Comparative genomics of the FtsK–HerA superfamily of pumping ATPases: implications for the origins of chromosome segregation, cell division and viral capsid …

LM Iyer, KS Makarova, EV Koonin… - Nucleic acids …, 2004 - academic.oup.com
Recently, it has been shown that a predicted P-loop ATPase (the HerA or MlaA protein),
which is highly conserved in archaea and also present in many bacteria but absent in …

Origin of the cell nucleus, mitosis and sex: roles of intracellular coevolution

T Cavalier-Smith - Biology direct, 2010 - Springer
Background The transition from prokaryotes to eukaryotes was the most radical change in
cell organisation since life began, with the largest ever burst of gene duplication and novelty …

Recombination proteins and rescue of arrested replication forks

B Michel, H Boubakri, Z Baharoglu, M LeMasson… - DNA repair, 2007 - Elsevier
Recombination proteins play crucial roles in the rescue of inactivated replication forks in
Escherichia coli. The enzymes that catalyze the repair of DNA double-strand breaks by a …

Xer site specific recombination: double and single recombinase systems

F Castillo, A Benmohamed, G Szatmari - Frontiers in microbiology, 2017 - frontiersin.org
The separation and segregation of newly replicated bacterial chromosomes can be
constrained by the formation of circular chromosome dimers caused by crossing over during …

Evidence for functional overlap among multiple bacterial cell division proteins: compensating for the loss of FtsK

B Geissler, W Margolin - Molecular microbiology, 2005 - Wiley Online Library
In Escherichia coli, at least 12 proteins colocalize to the cell midpoint, assembling into a
membrane‐associated protein machine that forms the division septum. Many of these …

The rarA gene as part of an expanded RecFOR recombination pathway: Negative epistasis and synthetic lethality with ruvB, recG, and recQ

K Jain, EA Wood, MM Cox - PLoS genetics, 2021 - journals.plos.org
The RarA protein, homologous to human WRNIP1 and yeast MgsA, is a AAA+ ATPase and
one of the most highly conserved DNA repair proteins. With an apparent role in the repair of …

Anticipating chromosomal replication fork arrest: SSB targets repair DNA helicases to active forks

F Lecointe, C Sérèna, M Velten, A Costes… - The EMBO …, 2007 - embopress.org
In bacteria, several salvage responses to DNA replication arrest culminate in reassembly of
the replisome on inactivated forks to resume replication. The PriA DNA helicase is a …

Requirements for catalysis in the Cre recombinase active site

B Gibb, K Gupta, K Ghosh, R Sharp, J Chen… - Nucleic acids …, 2010 - academic.oup.com
Members of the tyrosine recombinase (YR) family of site-specific recombinases catalyze
DNA rearrangements using phosphoryl transfer chemistry that is identical to that used by the …