Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …

Directed evolution of stereo‐, regio‐, and chemoselective enzymes constitutes a unique way
to generate biocatalysts for synthetically interesting transformations in organic chemistry and …

We report the engineering of mScarlet, a truly monomeric red fluorescent protein with record
brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns). We developed mScarlet …

Directed evolution has advanced into a standard industrial “tool” to tailor naturally occurring
proteins for a variety of biotechnological applications, thus enabling product valorization and …

The amino acid sequence of a protein affects both its structure and its function. Thus, the
ability to modify the sequence, and hence the structure and activity, of individual proteins in …

Thermostability is an essential requirement of enzymes in the industrial processes to
catalyze the reactions at high temperatures; thus, enzyme engineering through directed …

Enzymes generated by natural recruitment and protein engineering have greatly contribute
in various sets of applications. However, their insufficient stability is a bottleneck that limit the …

Directed evolution is one of the most powerful tools for protein engineering and functions by
harnessing natural evolution, but on a shorter timescale. It enables the rapid selection of …

Directed evolution has matured to a routinely applied algorithm to tailor enzyme properties
to meet the demands in various applications. In order to free directed enzyme evolution from …

Owing to the environmental friendliness and vast advantages that enzymes offer in the
biotechnology and industry fields, biocatalysts are a prolific investigation field. However, the …