Zebrafish: A complete animal model to enumerate the nanoparticle toxicity

C Chakraborty, AR Sharma, G Sharma… - Journal of …, 2016 - Springer
Presently, nanotechnology is a multi-trillion dollar business sector that covers a wide range
of industries, such as medicine, electronics and chemistry. In the current era, the commercial …

Zebrafish models of neurodevelopmental disorders: past, present, and future

C Sakai, S Ijaz, EJ Hoffman - Frontiers in molecular neuroscience, 2018 - frontiersin.org
Zebrafish are increasingly being utilized as a model system to investigate the function of the
growing list of risk genes associated with neurodevelopmental disorders. This is due in large …

The zebrafish reference genome sequence and its relationship to the human genome

K Howe, MD Clark, CF Torroja, J Torrance, C Berthelot… - Nature, 2013 - nature.com
Zebrafish have become a popular organism for the study of vertebrate gene function,. The
virtually transparent embryos of this species, and the ability to accelerate genetic studies by …

High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9

GK Varshney, W Pei, MC LaFave, J Idol, L Xu… - Genome …, 2015 - genome.cshlp.org
The use of CRISPR/Cas9 as a genome-editing tool in various model organisms has
radically changed targeted mutagenesis. Here, we present a high-throughput targeted …

An Indexed, Mapped Mutant Library Enables Reverse Genetics Studies of Biological Processes in Chlamydomonas reinhardtii

X Li, R Zhang, W Patena, SS Gang, SR Blum… - The Plant …, 2016 - academic.oup.com
The green alga Chlamydomonas reinhardtii is a leading unicellular model for dissecting
biological processes in photosynthetic eukaryotes. However, its usefulness has been limited …

[HTML][HTML] Vector design influences hepatic genotoxicity after adeno-associated virus gene therapy

RJ Chandler, MC LaFave, GK Varshney… - The Journal of …, 2015 - Am Soc Clin Investig
The use of adeno-associated virus (AAV) as a gene therapy vector has been approved
recently for clinical use and has demonstrated efficacy in a growing number of clinical trials …

The Zebrafish Information Network: new support for non-coding genes, richer Gene Ontology annotations and the Alliance of Genome Resources

L Ruzicka, DG Howe, S Ramachandran… - Nucleic acids …, 2019 - academic.oup.com
Abstract The Zebrafish Information Network (ZFIN)(https://zfin. org/) is the database for the
model organism, zebrafish (Danio rerio). ZFIN expertly curates, organizes and provides a …

Loss-of-function genetic tools for animal models: cross-species and cross-platform differences

BE Housden, M Muhar, M Gemberling… - Nature Reviews …, 2017 - nature.com
Our understanding of the genetic mechanisms that underlie biological processes has relied
extensively on loss-of-function (LOF) analyses. LOF methods target DNA, RNA or protein to …

A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish

GK Varshney, B Carrington, W Pei, K Bishop, Z Chen… - Nature protocols, 2016 - nature.com
The zebrafish is a popular model organism for studying development and disease, and
genetically modified zebrafish provide an essential tool for functional genomic studies …

Expanding the CRISPR toolbox in zebrafish for studying development and disease

K Liu, C Petree, T Requena, P Varshney… - Frontiers in cell and …, 2019 - frontiersin.org
The study of model organisms has revolutionized our understanding of the mechanisms
underlying normal development, adult homeostasis, and human disease. Much of what we …