Culture and differentiation of male germ cells has been performed for various purposes in
the past. To date, none of the studies aimed at in vitro spermatogenesis has resulted in a …

In vitro culture of spermatogonial stem cells (SSCs) has generally been performed using two-
dimensional (2D) culture systems; however, such cultures have not led to the development …

Establishment of an in vitro system that allows the development of testicular germ cells to
sperm will be valuable for studies of spermatogenesis and future treatments for male …

Isolation and culture of spermatogonial stem cells (SSCs) has become an approach to study
the milieu and the factors controlling their expansion and differentiation. Traditional …

Men's reproductive health exclusively depends on the appropriate maturation of certain
germ cells known as sperm. Certain illnesses, such as Klinefelter syndrome, cryptorchidism …

Spermatogenesis is contingent upon hormones and growth factors acting through endocrine
and paracrine pathways either in vivo or in vitro. Sertoli cells (SCs) furnish essential factors …

Achieving mammalian spermatogenesis in vitro has a long history of research but remains
elusive. The organ culture method has advantages over the cell culture method, because …

Pregnancies achieved by assisted reproduction technologies, particularly by
intracytoplasmic sperm injection (ICSI) procedures, are susceptible to genetic risks inherent …

Background: Testicular germ cell tumours of young adults, seminoma or non-seminomas,
are preceded by a pre-invasive precursor, carcinoma in situ (CIS), understood to arise …

OBJECTIVE: To assess the effectiveness of the three-dimensional culture of spermatogenic
cells in a collagen gel matrix from nonobstructive azoospermic patients and examine the …