While often obvious for macroscopic organisms, determining whether a microbe is dead or
alive is fraught with complications. Fields such as microbial ecology, environmental health …

Nucleic acid-based analytical methods, ranging from species-targeted PCRs to
metagenomics, have greatly expanded our understanding of microbiological diversity in …

The ideal scenario in most applications of microbial diagnostics is that only viable cells are
detected. Bacteria were traditionally considered viable when they could be cultured …

The application of beneficial, plant‐associated microorganisms is a sustainable approach to
improving crop performance in agriculture. However, microbial inoculants are often …

The limitation of 16S rRNA gene sequencing (DNA-based) for microbial community
analyses in water is the inability to differentiate live (dormant cells as well as growing or non …

Biofilms constitute the prevalent way of life for microorganisms in both natural and man-
made environments. Biofilm-dwelling cells display greater tolerance to antimicrobial agents …

The increase in foodborne outbreaks highlights the need for rapid, sensitive and specific
methods for food safety monitoring, enabling specific detection and quantification of viable …

The detection, enumeration, and virulence potential of viable but non-culturable (VBNC)
pathogens continues to be a topic of discussion. While there is a lack of definitive evidence …

Thanks to the achievements in sanitation, hygiene practices, and antibiotics, we have
considerably improved in our ongoing battle against pathogenic bacteria. However, with our …

Waterborne diseases have emerged as global health problems and their rapid and sensitive
detection in environmental water samples is of great importance. Bacterial identification and …