Vitamin B 12, cobalamin, is a cobalt-containing ring-contracted modified tetrapyrrole that
represents one of the most complex small molecules made by nature. In prokaryotes it is …

Seattle, Washington 98195 Changeux—are better established for NO/O2. This hemoglobin-
based paradigm for protein regulation (JSS, Two signaling systems, based on the principle …

From reviews to the first edtion:" Bornscheuer and Kazlauskas have set out, and succeeded,
in producing a definitive manual on hydrolytic enzymes (especially lipases, esterases, and …

Protein folding is a biophysical process by which proteins reach a specific three-dimensional
structure. The amino acid sequence of a polypeptide chain contains all the information …

While it has been known for more than 20 years that unusually stable cysteine-sulfenic acid
(Cys-SOH) derivatives can be introduced in selected proteins by mild oxidation, only …

Nitrile hydratase (NHase, EC 4.2. 1.84) is one of the key enzymes of nitrile metabolism in a
large number of microbes that catalyses the hydration of nitriles to corresponding amides …

Non-heme iron enzymes promote a number of important biological reactions, including
serotonin, 1 leukotreine, 2 and DNA3 synthesis. Most mononuclear non-heme iron enzymes …

Protein metal-occupancy (metalation) in vivo has been elusive. To address this challenge,
the available free energies of metals have recently been determined from the responses of …

Background COG0523 proteins are, like the nickel chaperones of the UreG family, part of the
G3E family of GTPases linking them to metallocenter biosynthesis. Even though the first …

Nitrile hydratase from Rhodococcus sp. N-771 is an αβ heterodimer with a nonheme ferric
iron in the catalytic center. In the catalytic center, αCys112 and αCys114 are modified to a …