MicroRNAs: a puzzling tool in cancer diagnostics and therapy

B D'ANGELO, E Benedetti, A Cimini… - Anticancer …, 2016 - ar.iiarjournals.org
MicroRNAs (miRNAs) constitute a dominating class of small RNAs that regulate diverse
cellular functions. Due the pivotal role of miRNAs in biological processes, a deregulated …

Dicer-TRBP complex formation ensures accurate mammalian microRNA biogenesis

RC Wilson, A Tambe, MA Kidwell, CL Noland… - Molecular cell, 2015 - cell.com
RNA-mediated gene silencing in human cells requires the accurate generation of∼ 22 nt
microRNAs (miRNAs) from double-stranded RNA substrates by the endonuclease Dicer …

MicroRNAs and human diseases: diagnostic and therapeutic potential

R Maqbool, MU Hussain - Cell and tissue research, 2014 - Springer
MicroRNAs (miRNAs) are endogenous, non-coding small RNAs that regulate gene
expression at the post-transcriptional level. Recent studies have shown that miRNAs are …

TRBP ensures efficient Dicer processing of precursor microRNA in RNA-crowded environments

M Fareh, KH Yeom, AC Haagsma, S Chauhan… - Nature …, 2016 - nature.com
The RNA-binding protein TRBP is a central component of the Dicer complex. Despite a
decade of biochemical and structural studies, the essential functionality of TRBP in …

Derivation and characterization of Dicer-and microRNA-deficient human cells

HP Bogerd, AW Whisnant, EM Kennedy, O Flores… - Rna, 2014 - rnajournal.cshlp.org
We have used genome editing to generate inactivating deletion mutations in all three copies
of the dicer (hdcr) gene present in the human cell line 293T. As previously shown in murine …

Down-regulation of the antisense mitochondrial non-coding RNAs (ncRNAs) is a unique vulnerability of cancer cells and a potential target for cancer therapy

S Vidaurre, C Fitzpatrick, VA Burzio, M Briones… - Journal of Biological …, 2014 - ASBMB
Hallmarks of cancer are fundamental principles involved in cancer progression. We propose
an additional generalized hallmark of malignant transformation corresponding to the …

Structural basis of si RNA recognition by TRBP double‐stranded RNA binding domains

G Masliah, C Maris, SLB König, M Yulikov… - The EMBO …, 2018 - embopress.org
The accurate cleavage of pre‐micro (mi) RNA s by Dicer and mi/si RNA guide strand
selection are important steps in forming the RNA‐induced silencing complex (RISC). The …

[图书][B] Sedimentation velocity analytical ultracentrifugation: interacting systems

P Schuck, H Zhao - 2017 - books.google.com
Analytical ultracentrifugation is one of the most powerful solution techniques for the study of
macromolecular interactions, to define the number and stoichiometry of complexes formed …

Dissecting the roles of TRBP and PACT in double‐stranded RNA recognition and processing of noncoding RNAs

A Heyam, D Lagos, M Plevin - Wiley Interdisciplinary Reviews …, 2015 - Wiley Online Library
HIV TAR RNA‐binding protein (TRBP) and Protein Activator of PKR (PACT) are double‐
stranded (ds) RNA‐binding proteins that participate in both small regulatory RNA biogenesis …

[HTML][HTML] Structural studies of RNA-protein complexes: A hybrid approach involving hydrodynamics, scattering, and computational methods

TR Patel, G Chojnowski, A Koul, SA McKenna… - Methods, 2017 - Elsevier
The diverse functional cellular roles played by ribonucleic acids (RNA) have emphasized
the need to develop rapid and accurate methodologies to elucidate the relationship between …