Long-read technologies are overcoming early limitations in accuracy and throughput,
broadening their application domains in genomics. Dedicated analysis tools that take into …

Identifying structural variation (SV) is essential for genome interpretation but has been
historically difficult due to limitations inherent to available genome technologies. Detection …

Forty years ago the advent of Sanger sequencing was revolutionary as it allowed complete
genome sequences to be deciphered for the first time. A second revolution came when next …

Since the completion of the human genome project in 2003, extraordinary progress has
been made in genome sequencing technologies, which has led to a decreased cost per …

While metagenomics has emerged as a technology of choice for analyzing bacterial
populations, the assembly of metagenomic data remains challenging, thus stifling biological …

While genome assembly projects have been successful in many haploid and inbred
species, the assembly of noninbred or rearranged heterozygous genomes remains a major …

Cultivated peanut (Arachis hypogaea) is an allotetraploid with closely related subgenomes
of a total size of∼ 2.7 Gb. This makes the assembly of chromosomal pseudomolecules very …

Background: Given the demonstrated utility of Third Generation Sequencing [Pacific
Biosciences (PacBio) and Oxford Nanopore Technologies (ONT)] long reads in many …

Long-read, single-molecule real-time (SMRT) sequencing is routinely used to finish
microbial genomes, but available assembly methods have not scaled well to larger …

DNA sequencing process utilizes biochemical methods in order to determine the correct
order of nucleotide bases in a DNA macromolecule using sequencing machines. Ten years …